FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_5.34100000006268.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_5.34100000006268.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482253
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA250.002105970435.99975228
AGGGCGA250.002105970435.99975210
TATGCCG950.030.78926343
GGTATCA2850.028.4296971
GTATCAA7000.027.9727941
GCCCACG1105.456968E-1226.59072518
CGAGACG801.0512122E-725.31232523
CGGGGGG551.3666591E-424.54528417
TGCCGTC1201.6370905E-1124.37483245
CCACGAG1400.024.10697620
CCCACGA1252.910383E-1123.39983719
CTCGTAT1204.4929038E-1022.49984639
GTAAGTA1800.022.4998462
ACGAGAC1305.0931703E-1122.49984622
TATCTCG855.022517E-621.17632536
ACGCCTA754.595767E-520.99985521
CGCCTAA754.595767E-520.99985522
CGAGCCC1401.364242E-1020.89271215
GTATAGA654.2246332E-420.775551
TCTCGTA1301.2223609E-920.76908938
Produced by FastQC (version 0.11.2)