FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_49.34100000006522.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_49.34100000006522.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317471
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAATA352.8158675E-432.14274629
TCCGTTC406.1563624E-428.12490316
TAATATT450.001224624624.99991231
ACCGGCC450.001224624624.9999128
GTAATAT450.001224624624.99991230
TTAGATG500.002261073822.4999223
ATTTTTA602.462114E-422.49992227
TACCGGC500.002261073822.4999227
TCTCCGA3700.021.89181345
ACTGAGC754.589294E-520.9999268
CGTCTAT654.224531E-420.7724291
ACCGGAA654.229026E-420.7691626
TACGGGC1301.2169039E-920.7691615
CGGGGCT654.229026E-420.7691617
TAGGTAG550.003930439220.45447332
CTATAAC550.003930439220.4544733
CTATTAA706.961018E-419.2886851
TAAGACT951.3109766E-518.9473024
TTGCGGT600.00650049318.74993317
TATAAGT600.00650049318.7499334
Produced by FastQC (version 0.11.2)