FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_48.34100000006515.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_48.34100000006515.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186142
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC105455.6650299233918195RNA PCR Primer, Index 46 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT2710.14558777707341708RNA PCR Primer, Index 18 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC11600.042.08825745
TATGCCG11750.041.93391843
ATGCCGT11700.041.92082644
CCGAATC11950.041.79691733
TCGTATG11900.041.7834740
CGAATCT12000.041.43527634
ATTCCGA12150.041.30517630
TCCGAAT12150.041.2940832
TCTCGTA12100.041.27877438
CGTATGC12000.041.24778741
CCACGAG12300.041.15632620
CATTCCG12200.041.1358929
CACGAGA12350.040.989721
CTCGTAT12200.040.94042239
AATCTCG12150.040.9237336
CGAGACC12350.040.8075223
GCCCACG12500.040.67781418
CCCACGA12450.040.6604719
ACGAGAC12450.040.6604722
ACCATTC12400.040.653927
Produced by FastQC (version 0.11.2)