Basic Statistics
Measure | Value |
---|---|
Filename | H23NVBCXX l02n01 24h_47.34100000006508.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 16309 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC | 7588 | 46.52645778404562 | RNA PCR Primer, Index 46 (95% over 21bp) |
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG | 595 | 3.648292353915016 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT | 326 | 1.9988963149181433 | No Hit |
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT | 64 | 0.3924213624379177 | RNA PCR Primer, Index 18 (95% over 21bp) |
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC | 48 | 0.2943160218284383 | No Hit |
CTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGCCC | 38 | 0.23300018394751365 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC | 30 | 0.18394751364277392 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC | 30 | 0.18394751364277392 | RNA PCR Primer, Index 39 (95% over 21bp) |
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC | 25 | 0.1532895947023116 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC | 24 | 0.14715801091421915 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC | 21 | 0.12876325954994175 | RNA PCR Primer, Index 45 (95% over 21bp) |
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC | 21 | 0.12876325954994175 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC | 21 | 0.12876325954994175 | No Hit |
ATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGCCCACG | 19 | 0.11650009197375683 | No Hit |
CCGTTGCAGTATCGATTCGCTCTGCATTTTTCTCAATCCATGAAGCCAAGG | 18 | 0.11036850818566435 | No Hit |
GCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT | 17 | 0.1042369243975719 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CATCTCG | 20 | 6.832682E-4 | 44.996933 | 36 |
TGCCGTC | 815 | 0.0 | 44.72088 | 45 |
ATGCCGT | 850 | 0.0 | 42.879433 | 44 |
GTATGCC | 915 | 0.0 | 39.833347 | 42 |
ATCTCGT | 915 | 0.0 | 39.833347 | 37 |
TATGCCG | 915 | 0.0 | 39.833347 | 43 |
TCTCGTA | 915 | 0.0 | 39.833347 | 38 |
CGTATGC | 915 | 0.0 | 39.833347 | 41 |
CTCGTAT | 915 | 0.0 | 39.833347 | 39 |
AATCTCG | 890 | 0.0 | 39.68831 | 36 |
GAATCTC | 885 | 0.0 | 39.658314 | 35 |
CGAATCT | 880 | 0.0 | 39.62798 | 34 |
TCGTATG | 920 | 0.0 | 39.616863 | 40 |
TTCCGAA | 875 | 0.0 | 39.5973 | 31 |
TCCGAAT | 875 | 0.0 | 39.5973 | 32 |
CCGAATC | 875 | 0.0 | 39.5973 | 33 |
CATTCCG | 895 | 0.0 | 38.712444 | 29 |
ATTCCGA | 895 | 0.0 | 38.712444 | 30 |
CCACGAG | 945 | 0.0 | 38.568798 | 20 |
CACGAGA | 945 | 0.0 | 38.568798 | 21 |