FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_47.34100000006508.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_47.34100000006508.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16309
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC758846.52645778404562RNA PCR Primer, Index 46 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG5953.648292353915016No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT3261.9988963149181433No Hit
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT640.3924213624379177RNA PCR Primer, Index 18 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC480.2943160218284383No Hit
CTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGCCC380.23300018394751365No Hit
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC300.18394751364277392No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC300.18394751364277392RNA PCR Primer, Index 39 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC250.1532895947023116No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC240.14715801091421915No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC210.12876325954994175RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC210.12876325954994175No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC210.12876325954994175No Hit
ATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGCCCACG190.11650009197375683No Hit
CCGTTGCAGTATCGATTCGCTCTGCATTTTTCTCAATCCATGAAGCCAAGG180.11036850818566435No Hit
GCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT170.1042369243975719No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTCG206.832682E-444.99693336
TGCCGTC8150.044.7208845
ATGCCGT8500.042.87943344
GTATGCC9150.039.83334742
ATCTCGT9150.039.83334737
TATGCCG9150.039.83334743
TCTCGTA9150.039.83334738
CGTATGC9150.039.83334741
CTCGTAT9150.039.83334739
AATCTCG8900.039.6883136
GAATCTC8850.039.65831435
CGAATCT8800.039.6279834
TCGTATG9200.039.61686340
TTCCGAA8750.039.597331
TCCGAAT8750.039.597332
CCGAATC8750.039.597333
CATTCCG8950.038.71244429
ATTCCGA8950.038.71244430
CCACGAG9450.038.56879820
CACGAGA9450.038.56879821