FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_46.341000000064fa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_46.341000000064fa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486862
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC26130.5367023920536005RNA PCR Primer, Index 46 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3200.038.67081545
ATGCCGT3750.032.99909644
CTCGTAT4050.030.55471839
TATGCCG4050.030.55471843
AATCTCG4200.028.39207836
TCCGAAT4300.028.2550432
CCGAATC4300.028.2550433
TCGTATG4400.028.12422840
CCCACGA4450.027.80822819
ATTCCGA4350.027.41585730
CCACGAG4650.027.09603120
TCTCGTA4600.026.90143638
CGAATCT4550.026.70256634
GACCATT4500.026.50199526
CGTATGC4700.026.32906541
ACGAGAC4700.026.32906522
CATTCCG4550.026.21076629
GCCCACG4750.026.05191818
ATCTCGT4700.025.85035537
CGAGACC4700.025.85035523
Produced by FastQC (version 0.11.2)