FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_45.341000000064ed.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_45.341000000064ed.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544838
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCCGA751.7007551E-929.99926830
AATCTCG1250.026.99933836
TATGCCG1600.026.71809643
TCGTATG1600.026.71809640
ATGCCGT1750.025.71365744
TGCCGTC1800.025.00168245
CGTATGC1750.024.42797541
CCCACGA1750.024.42797519
GCCCACG1800.023.74941818
GTATTGG802.9611656E-622.5077111
TGCGCAA500.002262208122.50151312
CGTCGTA500.002262208122.50151310
CTCGTAT1900.022.49944939
TCTCGTA1900.022.49944938
ACGAGAC1900.022.49944922
CCACGAG1950.021.9225420
CATTCCG1056.094524E-821.42804529
TCCGTCA550.003932386620.45592312
TCCGAAT1109.962605E-820.45404632
ACGATTT550.00393447820.4540469
Produced by FastQC (version 0.11.2)