FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_44.341000000064d0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_44.341000000064d0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences591345
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC10680.18060523044923013RNA PCR Primer, Index 46 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG1550.033.3850336
TATGCCG1900.033.15583843
GCCACGA352.819758E-432.14086518
GCCCACG2000.031.49804918
CCCACGA2000.031.49804919
ATTCCGA1400.030.53640430
TGCCGTC2000.030.37311745
ACGAGAC2100.030.00067722
CGAGACC1550.027.58126623
CCACGAG2500.027.0006120
CTCGTAT2250.026.99832739
CCGAATC1700.025.14549833
GTACGAT450.001223877225.0069081
CTGAGCG450.001225689225.00056520
TCGTATG2700.024.9984540
CACGAGA2550.024.70643821
ATGCCGT2550.024.7043544
TTCCGAA1750.024.42705531
TCTCGTA2500.024.29849438
CGAGCCC2600.024.22926515
Produced by FastQC (version 0.11.2)