FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_43.341000000064c3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_43.341000000064c3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440408
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC5910.13419374761584713RNA PCR Primer, Index 46 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1300.031.15620443
CGTATAG300.0051362430.0090871
TCGTATG1350.029.99886340
CCCACGA1450.029.48164219
CGAGCCC1350.028.33547815
CTCGTAT1450.027.92997739
ACGAGAC1550.027.57960122
ATGCCGT1500.027.00204544
TGCCGTC1550.026.1310145
GCCCACG1650.025.9110518
CCACGAG1600.025.3115420
AATCTCG1251.8189894E-1225.19904736
GGTATCA3450.024.7901131
GTATCAA7000.024.7574941
ATCTCGT1650.024.54452537
TCTCGTA1700.023.82262638
ACGGGAT1052.3555913E-923.57321416
CATTCCG1252.910383E-1123.39911529
CGTATGC1800.022.50170541
ACACCGA500.002261413522.50170342
Produced by FastQC (version 0.11.2)