FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_42.341000000064b6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_42.341000000064b6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511171
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC6720.13146285685220796RNA PCR Primer, Index 46 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGGG250.002106218835.99929411
TATGCCG1750.028.28515843
TCGTATG1800.027.49946240
ATGCCGT1800.027.49946244
CTCGTAT1900.026.05212239
TGCCGTC1900.026.05212245
GGTATCA5150.025.7963811
AATCTCG1850.025.54004136
CCCACGA2050.025.24340819
CGTATGC2000.024.74951641
TCTCGTA2050.024.14586838
ATTCCGA1750.023.14240330
CGAGCCC2200.022.4995615
CCACGAG2200.022.4995620
ACGAGAC2250.021.9995722
GTATCAA9950.021.9514221
GCCCACG2300.021.52131718
CACGAGA2300.021.52131721
CCGAGCC2300.021.52131714
ACCATTC2000.021.3745827
Produced by FastQC (version 0.11.2)