FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_4.3410000000625c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_4.3410000000625c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510859
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTTC300.005143519530.00130345
GGTATCA2250.029.0154611
AACCGTC957.403287E-1026.05121434
GTATCAA5600.025.7279971
GCGTGCT450.001226010524.9986411
CAACCGT952.1107553E-823.68292233
ACCGTCT1052.3610482E-923.57014735
GTATAAA702.6881018E-522.5119971
CCTAGAT602.4658145E-422.4987773
ACGTACA754.598133E-520.9988572
ACGCCTA1401.364242E-1020.89376621
ATATAAG1109.900941E-820.4654521
GTTACGG908.226461E-620.00282925
CATCTCC9250.019.94681445
ATCTCCG3500.019.92943845
ATATGTA2300.019.575651
ACAACCG1151.591925E-719.56415432
CGCCTAA1503.4197E-1019.50084922
TATCAAC7200.019.3739452
TACGTAC706.951216E-419.2959981
Produced by FastQC (version 0.11.2)