FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_39.34100000006480.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_39.34100000006480.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517299
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC15910.30755907125279575No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA1700.038.38218731
GGCCGAT250.002106083835.99984411
CTCGTAT2150.035.5812439
TATGCCG2150.035.5812443
TATCTCG2050.035.121836
CTGTCCG401.5589607E-533.74985510
ACCTATC2050.032.92668533
CCCACGA2400.032.8123619
TCTCGTA2350.032.55305538
TGCCGTC2350.032.55305545
TGCGACC1900.031.97354729
GCCCACG2400.031.87486318
TCGTATG2400.031.87486340
ACGAGAC2450.031.22435622
ATGCCGT2500.030.59986744
ACGCCTA453.487274E-529.9998721
CGTATGC2550.029.9998741
CGAGACT2350.029.68072523
CTGCGAC2050.029.63401828
GACCTAT2050.029.63401832
Produced by FastQC (version 0.11.2)