FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_38.34100000006473.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_38.34100000006473.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415987
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC20370.48967876399983656No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2900.036.4619145
CGACCTA3150.033.5681131
TATGCCG3250.033.22748643
GTTTACG352.8169501E-432.1435439
CGAGACT3300.032.04228623
ACCTATC3400.031.76156633
TCGTATG3400.031.76156640
ATGCCGT3400.031.76156644
ACGAGAC3400.031.76156622
TGCGACC3450.031.30501629
CTCGTAT3500.030.85409439
GCGACCT3450.030.65282830
CTGCGAC3450.030.65282828
GACTGCG3450.030.65282826
TATCTCG3550.030.41952936
GCCCACG3600.030.0006418
TCTCGTA3600.029.99703638
GTTTCGG300.005146012629.99703442
CCTAGAC300.005146012629.9970343
TAGGTTC300.005146012629.9970344
Produced by FastQC (version 0.11.2)