FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_37.34100000006466.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_37.34100000006466.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences546619
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1500.026.99912343
CCCACGA1700.025.1462419
GGATCGT551.3672223E-424.5446576
CCACGAG1800.023.75140220
CTCGTAT1750.023.14210539
TGCCGTC1850.023.10735745
ACGAGAC1800.022.50132822
TCGTATG1800.022.4992740
CGACCTA500.0022635322.49926831
CGCTTGC602.4657557E-422.49926819
CGAGACT1551.8189894E-1221.77547823
ATGCCGT2000.021.37430444
GGTATCA7300.020.9620571
CGAGCCC2050.020.85488715
TTCTAAT654.232628E-420.77045622
GTCCGAG908.234056E-620.0011811
CGTATGC2050.019.75545341
TCTCGTA2050.019.75545338
CTAAACT807.555625E-519.686864
CCGAGCC2100.019.28685214