FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_36.3410000000645a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_36.3410000000645a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences571567
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC7880.13786660181571014No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGCG207.0294994E-445.001055
TGCCGTC1600.030.93551645
CGACCTA1100.028.63452731
TATGCCG1750.026.99826843
GCCCACG1850.026.7550418
CGAGACT1800.026.25061223
ATGCCGT2000.024.74841144
GCGACCT1201.6370905E-1124.37556830
CTGCGAC1201.6370905E-1124.37556828
TGCGACC1201.6370905E-1124.37556829
GTACCGT651.5217347E-524.2313356
CCCACGA2050.024.14479319
GACTGCG1252.910383E-1123.40259226
GAGTAGT500.002258322222.5084021
GGCTAGC500.002258322222.5084021
TACCGCA702.6994352E-522.50052513
CCACGAG2250.022.00051320
CGAGCCC2300.021.5222415
TCCGTGT754.5966073E-521.0004928
TCGTATG2300.020.5421640
Produced by FastQC (version 0.11.2)