FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_35.3410000000644d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_35.3410000000644d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406069
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC19920.4905570235600354No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2800.038.5712245
ATGCCGT2900.037.24117744
TATGCCG3000.035.999843
GCGACCT2900.034.913630
TGCGACC2900.034.913629
CGACCTA2950.034.32184631
CTCGTAT3300.032.72709339
TCTCGTA3300.032.72709338
ACCTATC3100.032.6611133
TATCTCG3100.032.6611136
CGAGACT3200.032.34357523
GACTGCG3200.032.34357526
TCGTATG3350.032.2386340
ACGAGAC3350.032.2386322
CTGCGAC3150.032.1426828
GACCTAT3300.032.0452832
GCCCACG3450.031.30417818
CCCACGA3400.031.10277219
CACGAGA3450.030.65200821
ATCTCGT3500.030.2141237
Produced by FastQC (version 0.11.2)