FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_34.34100000006430.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_34.34100000006430.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496875
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC8350.1680503144654088No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1700.033.08690343
TCGTATG1900.030.78823540
CGGCTTA300.005142959330.00181214
GACCTAT1650.029.99879332
CTCGTAT1900.029.60407339
ATGCCGT1900.029.60407344
CGACCTA1750.029.5702431
ACCTATC1750.028.28457833
TGCGACC1750.028.28457829
TGCCGTC2000.028.12386745
CCACGAG2100.027.8560220
CGTATGC2050.027.4379241
CTGCGAC1850.026.7556828
GACTGCG1850.026.7556826
CGAGACT1950.026.53739423
GCGACCT1900.026.05158230
CGGGTGG1058.367351E-1125.71583717
CCCACGA2200.025.56715419
TCTCGTA2200.025.56715438
CACGAGA2300.025.43375821
Produced by FastQC (version 0.11.2)