FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_31.34100000006409.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_31.34100000006409.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2598
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC381.4626635873749037No Hit
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC261.0007698229407236No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC230.8852963818321785No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC220.8468052347959968No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC150.5773672055427251RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC130.5003849114703618No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC130.5003849114703618RNA PCR Primer, Index 46 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC110.4234026173979984No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC110.4234026173979984RNA PCR Primer, Index 39 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC100.3849114703618168No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC80.30792917628945343No Hit
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC80.30792917628945343No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC60.23094688221709006No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC50.1924557351809084RNA PCR Primer, Index 23 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC50.1924557351809084No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTC50.1924557351809084No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC50.1924557351809084No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC40.15396458814472672No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC30.11547344110854503No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGT30.11547344110854503No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG205.790163E-445.036
AGCCCAC301.431812E-644.99999617
GTATGCC301.431812E-644.99999642
ATCTCGT301.431812E-644.99999637
GCCCACG301.431812E-644.99999618
TGCCGTC301.431812E-644.99999645
CCACGAG301.431812E-644.99999620
TATGCCG301.431812E-644.99999643
GAGCCCA301.431812E-644.99999616
CACGAGA301.431812E-644.99999621
CCCACGA301.431812E-644.99999619
TCTCGTA301.431812E-644.99999638
ACGAGAC301.431812E-644.99999622
ATGCCGT301.431812E-644.99999644
CGTATGC301.431812E-644.99999641
CGAGCCC301.431812E-644.99999615
CCGAGCC301.431812E-644.99999614
TCGTATG301.431812E-644.99999640
CTCGTAT301.431812E-644.99999639
TCCGAGC354.142119E-638.57142613
Produced by FastQC (version 0.11.2)