FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_30.341000000063fb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_30.341000000063fb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545577
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC6590.1207895494128235No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1500.031.49935743
TGCCGTC1450.031.0338545
TCGTATG1550.030.48324840
AATCTCG1600.029.53064736
CTCGTAT1650.028.6357839
TCTCGTA1650.028.6357838
ACGAGAC1750.026.9994522
CGAGCCC1900.026.05210115
GGTATCA4450.025.2873381
CGTATGC1950.024.23027641
CCGAGCC1950.024.23027614
CGAGACC1900.023.6837323
ATGCCGT1900.023.6837344
CACGAGA2000.023.6245221
GTATCAA9100.023.4949931
GCCCACG2050.023.04831118
GAATCTC2100.022.49954235
CCCACGA2200.022.49954219
GTATGCC2300.020.5430642
ATCTCGT2450.020.2036737
Produced by FastQC (version 0.11.2)