FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_3.3410000000624f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_3.3410000000624f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319834
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGCAC504.8605216E-835.9996263
GCACCTA551.1234806E-732.7269336
GACTAGG453.4721572E-530.0137651
ATTATAC300.00514203129.9996873
GTATCAA3450.028.7088181
GTATTGG554.1377825E-628.6495041
CCGATTA554.1529293E-628.63606617
GTATAGT654.835438E-727.7050131
ATAGTTT551.3652854E-424.54526
TCCGATT651.5194095E-524.23051816
TTCGTTG651.5194095E-524.23051812
ACCCTAT651.5194095E-524.23051812
CCTCCGA651.5194095E-524.23051814
TCGTTGC751.6914182E-623.9997513
ATCTCCG1600.023.90645
CTAGAGT702.6953368E-522.4997673
GAGTAGC702.6953368E-522.4997676
CGATTAG702.6953368E-522.49976718
AGAGTAG702.6953368E-522.4997675
GTAGCTC702.6953368E-522.4997678
Produced by FastQC (version 0.11.2)