FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_29.341000000063ee.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_29.341000000063ee.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4364
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC1032.360219981668194No Hit
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC741.6956920256645278No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC741.6956920256645278No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC511.1686526122823098RNA PCR Primer, Index 39 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC451.0311640696608617RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC340.7791017415215399No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT220.5041246562786434No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC190.4353803849679193No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC120.27497708524289644RNA PCR Primer, Index 23 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT120.27497708524289644No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC100.229147571035747No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC90.2062328139321723No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC80.18331805682859761No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGT60.13748854262144822No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC60.13748854262144822No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC50.1145737855178735No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGT50.1145737855178735No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCG50.1145737855178735No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCAT253.2857166E-545.028
TTCATTA253.2857166E-545.030
ACGTTCA253.2857166E-545.027
GTTCATT253.2857166E-545.029
CATTAAT253.2857166E-545.032
GACGTTC253.2857166E-545.026
AGACGTT253.2857166E-545.025
ATTAATC253.2857166E-545.033
TCATTAA253.2857166E-545.031
TTAATCT253.2857166E-545.034
GAGACGT358.8202796E-845.024
CCGAGCC800.042.187514
TCCGAGC800.042.187513
ACGAGAC700.041.78571322
AATCTCG506.475602E-1040.536
CGAGACG451.2822056E-840.023
AGCCCAC850.039.70588317
GCCCACG850.039.70588318
CCACGAG850.039.70588320
CATCTCC850.039.7058839
Produced by FastQC (version 0.11.2)