FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_28.341000000063d1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_28.341000000063d1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500975
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC7790.15549678127651081No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT2050.031.82842344
TGCCGTC2050.031.82842345
TATGCCG2100.031.070643
TCTCGTA2250.028.99922638
AATCTCG1800.028.74923536
GCCCACG2300.028.37164118
CTCGTAT2350.027.76521939
CACGAGA2450.027.55028721
ATCTCGT2500.026.09930437
CCCACGA2500.026.09930419
TCGTATG2500.026.09930440
ACGAGAC2500.026.09930422
CCACGAG2500.026.09930420
CGAGACC1900.026.05193723
CGAGCCC2850.024.47547715
CGTATGC2700.024.16602341
CTATTCC952.1100277E-823.683584
CGCCATA500.002263190722.49940129
CGCCTAA754.5969202E-520.99944122
ACCTTGC2150.020.92967427
Produced by FastQC (version 0.11.2)