FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_27.341000000063c4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_27.341000000063c4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480562
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7650.029.7225151
TGTAGGG755.628317E-826.9982512
ATGCCGT709.293908E-725.7126244
GTGTAGG1152.6193447E-1023.4914071
GTATCAA14900.023.2680551
TACTTCG500.002260822322.50322516
CTCGTAT903.2807475E-722.49854539
TCGTATG903.2807475E-722.49854540
CACGCTT602.465691E-422.49854323
TATGCCG802.9704515E-622.4985443
CCCTTAC654.2173214E-420.7808591
GTCCTAT550.00392052320.4659981
ATGTAAG550.00392052320.4659981
TATCAAC17600.019.6862262
CACGGGA600.00650754318.74878523
CTCTAAA600.00650754318.7487852
GTAGGGC851.2031878E-418.5282123
TAACGAG1102.247345E-618.4117316
AACGCAG18950.018.4044176
CAACGCA18900.018.3340555
Produced by FastQC (version 0.11.2)