FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_25.341000000063ab.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_25.341000000063ab.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288528
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA54611.8927105861476181No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA46771.6209865247047082No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC38731.3423307270004992No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG38011.3173764764598237No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATAC34111.1822076193644984No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG33001.1437364831142904No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG32071.1115039094992514No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATAC29721.0300560084289911No Hit
GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCG27910.9673237952642377No Hit
GATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAG26050.9028586480341596No Hit
TATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGAG20060.6952531470082626No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATACA20030.6942133865690678No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATA19630.6803499140464704No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAACTGTCTCTTATA18560.6432651250485222No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATA16880.5850385404536128No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACA16710.5791465646315089No Hit
TGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC15300.5302778239893529No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATA14980.519187045971275No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT14730.5105223756446514No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC14040.48660788554317086No Hit
TGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGA13350.4626933954416902No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG13240.4588809404979759No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCC13130.4550684855542616No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATC12410.4301142350135862No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCCG12130.420409804247768No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT12080.4186768701824433No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATCT11560.4006543559030666No Hit
TATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCGA11530.39961459546387185No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAACTGTCTCTTAT11380.3944157932678978No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATCT10790.37396717129706647No Hit
GATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCC10700.37084788997948204No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC10440.36183663283979367No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAACTGTCTCTTATAC10280.3562912438307547No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC10090.3497060943825209No Hit
ATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA9780.3389619031775079No Hit
TTTTTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTAT9770.338615316364443No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAACTGTCTCTTAT9510.3296040592247546No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCC9370.3247518438418455No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCCTGTCTCTTATACACATCTCC9130.316433760328287No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAACTGTCTCTTAT9010.3122747185715078No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATAC8970.31088837131924807No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAACTGTCTCTTA8660.300144180114235No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8570.2970248987966506No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATCTC8280.2869738812177674No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCTGTCTCTTATACACATCTCCGA8160.2828148394609882No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAACTGTCTCTTATA8100.28073531858259854No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA7580.26271280430322186No Hit
TTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTAT7510.2602866966117673No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7290.2526617867243387No Hit
GTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTC7160.24815615815449452No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6820.2363722065102867No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAACTGTCTCTTATACA6410.22216214717462432No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAACTGTCTCTTA6400.22181556036155936No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACCTGTCTCTTATACACATCTC6350.22008262629623468No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTAT6260.21696334497865025No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAACTGTCTCTT6070.21037819553041648No Hit
GTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC5980.20725891421283205No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCG5920.2051793933344424No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATC5880.20379304608218268No Hit
GATACCACTGCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTC5860.2030998724560528No Hit
TTTTTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACTGTCTCTTATA5820.20171352520379307No Hit
CGTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACAT5760.1996340043254034No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACCTGTCTCTTATACACATC5700.19755448344701382No Hit
GATACCACCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA5670.196514723007819No Hit
GATACCACTGCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCG5550.19235568125103977No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAACTGTCTCTTATA5510.19096933399878002No Hit
GCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT5490.19027616037265016No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATCTGTCTCTTATACACATCTCC5370.1861171186158709No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAGAAAACTGTCTCTTATA5310.18403759773748127No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATACAC5270.18265125048522154No Hit
GATACCACCCCGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTC5250.18195807685909168No Hit
GGTATCAACGCAGAGTACGGGTGGTATCCTGTCTCTTATACACATCTCCGA5180.1795319691676371No Hit
TTTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTA5160.17883879554150722No Hit
ATACCACTGCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGA5130.17779903510231243No Hit
TTTTTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTTATACA5070.1757195142239228No Hit
TTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCC5000.17329340653246825No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAACTGTCTCTTATAC4960.1719070592802085No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAACTGTCTCTTAT4960.1719070592802085No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACAT4930.1708672988410137No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCCTGTCTCTTATACACATCTC4930.1708672988410137No Hit
GATACCACCCCGTACTCTGCGTTGATCTGTCTCTTATACACATCTCCGAGC4920.17052071202794875No Hit
TGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTC4910.17017412521488381No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATC4850.1680946043364942No Hit
CGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG4840.16774801752342927No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCCTGTCTCTTATACACATCTCCG4840.16774801752342927No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC4820.1670548438972994No Hit
TTTTCTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATA4710.16324238895358512No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATCTCC4700.16289580214052016No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4660.1615094548882604No Hit
GTATCAACGCAGAGTACGGGGCAGTGGTATCCTGTCTCTTATACACATCTC4650.16116286807519548No Hit
CGTTGATACCACTGCCCGTACTCTGCGTTGATACTGTCTCTTATACACATC4600.1594299340098708No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATCTGTCTCTTATACACATCTC4570.15839017357067597No Hit
TTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATA4570.15839017357067597No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCCGA4550.1576969999445461No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACAT4510.15631065269228636No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCACTGTCTCTTATACACATCTC4500.15596406587922143No Hit
GCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC4490.1556174790661565No Hit
GTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCC4400.15249819774857204No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT4400.15249819774857204No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACTGTCTCTTATACACATCTCC4390.15215161093550714No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCTGTCTCTTATACACAT4380.15180502412244218No Hit
GATACCACTGCCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTC4380.15180502412244218No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAACTGTCTCTTA4310.14937891643098763No Hit
TTTTTTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTA4290.14868574280485775No Hit
GTTGATACCACTGCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT4270.14799256917872788No Hit
GTGGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTC4250.14729939555259802No Hit
ATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGAGC4250.14729939555259802No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCCTGTCTCTTATACACATCT4250.14729939555259802No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATCTC4190.1452198746742084No Hit
TTTTTGCGTTGATACCACCCGTACTCTGCGTTGATACCCTGTCTCTTATAC4170.14452670104807852No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATCTGTCTCTTATACAC4160.1441801142350136No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACCTGTCTCTTATACACATCTCC4160.1441801142350136No Hit
ATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAG4100.14210059335662398No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4030.1396744856651694No Hit
GTATCAACGCAGAGTACGGGGTGGTATCTGTCTCTTATACACATCTCCGAG3990.13828813841290966No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACCTGTCTCTTATACACATCT3860.13378250984306547No Hit
GATACCACCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAG3860.13378250984306547No Hit
ACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC3860.13378250984306547No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAACTGTCTCTTA3830.13274274940387068No Hit
TTTTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTT3790.13135640215161093No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCTGTCTCTTATACACATCTCCG3730.12927688127322132No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3730.12927688127322132No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC3700.1282371208340265No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAACTGTCTCTTA3680.12754394720789664No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAACTGTCTCTTAT3670.1271973603948317No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAACTGTCTCTTATACAC3660.12685077358176675No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAACTGTCTCTTATAC3640.12615759995563688No Hit
TGATACCACCCCGTACTCTGCGTTGATCTGTCTCTTATACACATCTCCGAG3590.1244246658903122No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCTGTCTCTTATACACATC3560.1233849054511174No Hit
TTTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTAT3510.12165197138579271No Hit
CGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTC3440.11922586369433816No Hit
ACCCCGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC3440.11922586369433816No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA3400.1178395164420784No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAACTGTCTCTTATACA3380.11714634281594855No Hit
ATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCGAG3380.11714634281594855No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAGAAAAACTGTCTCTTAT3360.11645316918981867No Hit
GCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCC3330.11541340875062386No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAACTGTCTCT3290.11402706149836411No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAGAAAACTGTCTCTTATAC3250.11264071424610436No Hit
GTATCAACGCAGAGTACGGGTGGTATCCTGTCTCTTATACACATCTCCGAG3200.11090778018077968No Hit
GTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCT3190.11056119336771474No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCCTGTCTCTTATACACATCTC3180.11021460655464982No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACCTGTCTCTTATACACATCT3180.11021460655464982No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACCTGTCTCTTATACACATCTCCG3120.10813508567626018No Hit
TTTTTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACCCTGTCTCTTA3050.10570897798480564No Hit
GATACCACTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG2990.10362945710641602No Hit
TTTTTTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTTATAC2950.10224310985415627No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3600.044.9994845
ATGCCGT4100.039.51173844
GTATGCC4450.036.40407642
TATGCCG4450.036.40407643
CGTATGC4450.036.40407641
GAAAAGT250.002099252336.018311
AATCTCG4500.035.99958436
GAATCTC4500.035.99958435
TCGTATG4500.035.99958440
AGAATCT4450.035.8984634
CAGAATC4450.035.8984633
CTCGTAT4600.035.7061139
TGCAGAA4500.035.49958831
TCTCGTA4650.035.32217438
CCTTGCA4600.035.21698428
CTTGCAG4600.035.21698429
GCAGAAT4550.035.1094832
TTGCAGA4650.034.8383130
ACCTTGC4750.034.5785527
AGACCTT4800.034.21835325
Produced by FastQC (version 0.11.2)