FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_23.34100000006381.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_23.34100000006381.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9114
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC253627.825323677858236No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG2202.413868773315778No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT971.064296686416502No Hit
CTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGCCC480.5266622778143515No Hit
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC390.4279131007241606No Hit
TTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT390.4279131007241606RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC280.30721966205837176No Hit
GACGATGAAGCTTTGGTACAAATCGAGCCTGCTACTGCTTTCCCAAGTAAA230.2523590081193768No Hit
GCAGCGTCAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACG190.20847048496818082No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC180.19749835418038184No Hit
ACCCCGATATTGAAAAACTCAGGGATTTCCCATTGCCATTGTGGATAAGGG180.19749835418038184No Hit
TCAAGGCAAGCTCTTGATGGATCGAGATTATTGGTTTATTCCATGGATCAA170.18652622339258285No Hit
GTACATCTCCGACAAGTGCTGTTGAATCTCACGTACTGTCATGCCTCTGGC160.17555409260478386No Hit
GAAGTCGTTCATGGCACTTGGGATTCGACCGTCATCAAGTTACGAGAGAGT150.16458196181698487No Hit
GATGTTGTGCCTGTTTTAGGGGTGGGTGCGAATTACAATCTGACCAAAAAT150.16458196181698487No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC150.16458196181698487No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC150.16458196181698487RNA PCR Primer, Index 39 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC150.16458196181698487No Hit
ACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGCCCACGAG140.15360983102918588No Hit
GCAGCAATCGAGCCAATACAGAGATGGAAGGTGGTCATTTCAAATCTTCTA140.15360983102918588No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC140.15360983102918588No Hit
GGCATTAGGTGTTGCTTCTTTAGAAGAAGGTATCATGCTGCGCGTCTCAGG130.14263770024138686No Hit
CATTGGCTACTTTAACCACCTTAAAGCGGACTCCAGGAATATCACCTACAG130.14263770024138686No Hit
CCCATACCCGGGCAAGCAATCGTCAGAAGACCAGATAATGAGCACATCACT130.14263770024138686No Hit
GGATGGATGGTTTTCTCATAAATCCTCCGTTCTCTTTCATTGCATTTACGC130.14263770024138686No Hit
GGTGTGTCCTCAGATAGAGGGCTTTGTGGTGCTATTCACTCCTCAGTGGCT130.14263770024138686No Hit
CAGCTCATACAGTTAGGAAAAGAAAATGGCAAAGGCAAATCAAGCAGTAAC130.14263770024138686No Hit
GCTAGTACTGTAGCTGGTAACCACAGCTGTAAGTTCTTCGCTAAAACGATT120.13166556945358787No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGC120.13166556945358787No Hit
CGTTAATGATTTCGATCAATTCCATTCGATGATTCCATTCGATTCCATTCG110.12069343866578888No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC110.12069343866578888RNA PCR Primer, Index 45 (95% over 21bp)
GAATAAGGCATCCTCAACAAACAGGTAATCCTGTTTACGGTTTTCATCCCA110.12069343866578888No Hit
GATACGATACAACGCAGCATGCTCATGCTTCATCAGAAACTCAGCAGCACA110.12069343866578888No Hit
CAGAAAAAGACGTATTTGTTGAGCTTAATTTGTTCACCGGTATCCTGATCG110.12069343866578888No Hit
ATCATCACTGATCTCCTTCTCCCGTTCCTTCTCCAAATAGAGGGTGATGGG110.12069343866578888No Hit
GTTGGCCGATTACCTGGATACAGACCGCATCATCATCCAGACCGACGATAT110.12069343866578888No Hit
TCGCGGCAGCACTTTCATTCGATAAAACCATGTCACTCTACGAGCTGTCTC110.12069343866578888No Hit
TACACAGACATACCAGCCGTATAACAAAGACTGGATCAAAGAGAAGATCTA100.10972130787798992No Hit
GTATTGCGTGCCAATGTCATTACCTTGGCGGTTTAAGGTGGTAGGGTCATG100.10972130787798992No Hit
AGCCTCCACTTTATCGCTAACCAATCTATATGTTTCGTTGAAAGCTAAAAC100.10972130787798992No Hit
GGATAGTAGGTTGAACTGGTAGCTGCAGGCGTTGTTGTCGTTTGAGAGCTT100.10972130787798992No Hit
ATCCTAACTACCTGATGAACTTGAGCCCTAACAGCATGGATAGTGTGCGGC100.10972130787798992No Hit
CTCATTGTCTATGCTCATCCATAGCCGTTCACTTGGGAACCTGTCTCTTAT100.10972130787798992No Hit
GCCTAAGAGTGCTCGAAATGTCCATTTCCACATACTCCACAAAGTGTGTTT100.10972130787798992No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC100.10972130787798992No Hit
GTTACAGCAAGCATTAGCCAGTGAGGTGGATTACTTGAGGATTGCAAGCGG100.10972130787798992No Hit
AGTGTGGTTTGAGCGCAATGAATACAGTGGTGATTTAGTGCCGGCGCTGCG100.10972130787798992No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2850.045.045
ATGCCGT3000.042.7544
GTATGCC3400.037.7205942
TATGCCG3400.037.7205943
CGTATGC3400.037.7205941
TCGTATG3450.037.17391240
CTCGTAT3400.037.05882339
ATCTCGT3350.036.94029637
TCTCGTA3350.036.94029638
GATCCTA3200.036.562531
AGATCCT3200.036.562530
TATCTCG3150.036.42857436
ATCCTAT3100.036.2903232
CCTATCT3050.036.1475434
TCCTATC3050.036.1475433
CTATCTC3050.036.1475435
TAGATCC3250.035.99999629
CGAGACT3600.035.023
CCACGAG3750.034.820
CACGAGA3750.034.821
Produced by FastQC (version 0.11.2)