FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_21.34100000006367.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_21.34100000006367.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530628
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4500.032.5145031
CTCGTAT751.6951471E-623.99939739
GTATCAA8800.022.510041
TGTGCGC702.6999756E-522.49943510
TATGCCG802.9703988E-622.49943443
CATAACC550.0039343620.45403114
CTCCGAG3300.019.0904345
TCGTATG951.3139366E-518.94689240
ATCTCCG8100.018.88841445
CTAGACA600.00650692918.7495274
CTAATAC600.00650692918.7495273
CCTAGAC851.203122E-418.5289463
CTATTCT750.0011093417.9995484
CATCTCC18600.017.90277745
TCTCGTA1053.1079897E-517.14242738
TTTACAC800.001709494516.8745753
TATCAAC11750.016.6591572
CGGGGGG952.8163084E-416.57853118
CAGTCCG1104.6309484E-516.3632269
CACGAGA1257.612476E-616.19959321
Produced by FastQC (version 0.11.2)