FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_20.3410000000635b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_20.3410000000635b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences303300
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC9770.32212331025387403No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG4970.16386416089680186No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAATA207.026334E-444.99835212
ATACCCG352.8161216E-432.141687
TGCCGTC1600.030.93636745
CGAGCAC300.00514227729.9989013
ATGCCGT1950.025.38368444
TATGCCG2150.023.02241343
CCTACCC702.695291E-522.4991763
ACTAATC702.695291E-522.49917621
CTCGTAT2300.022.49917439
TCGTATG2350.022.0204740
GTATTGG654.2152693E-420.7787481
GGAGCCA654.2293718E-420.768475
TACCCGT550.003930797320.4537948
TAGGCAG550.003930797320.4537945
CCCACGA2550.020.29337319
TCTCGTA2550.020.29337338
ACGAGAC2550.020.29337322
CACGAGA2800.020.0885521
ATCTCGT2600.019.90311837
GCCCACG2600.019.90311818
Produced by FastQC (version 0.11.2)