##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 24h_2.34100000006232.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4634 Sequences flagged as poor quality 0 Sequence length 51 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.83966335779024 38.0 38.0 38.0 32.0 38.0 2 35.86965904186448 38.0 32.0 38.0 32.0 38.0 3 35.8256365990505 38.0 32.0 38.0 32.0 38.0 4 35.941950798446264 38.0 32.0 38.0 32.0 38.0 5 35.98036253776435 38.0 32.0 38.0 32.0 38.0 6 37.85261113508847 40.0 38.0 40.0 32.0 40.0 7 37.689684937419074 40.0 38.0 40.0 32.0 40.0 8 37.675442382391026 40.0 38.0 40.0 32.0 40.0 9 37.65041001294778 40.0 38.0 40.0 32.0 40.0 10 37.5953819594303 40.0 38.0 40.0 32.0 40.0 11 37.61480362537765 40.0 38.0 40.0 32.0 40.0 12 37.470867501078985 40.0 38.0 40.0 32.0 40.0 13 37.4717306862322 40.0 38.0 40.0 32.0 40.0 14 37.43547690979715 40.0 38.0 40.0 32.0 40.0 15 37.463746223564954 40.0 38.0 40.0 32.0 40.0 16 37.26694000863185 40.0 38.0 40.0 32.0 40.0 17 37.47302546396202 40.0 38.0 40.0 32.0 40.0 18 37.24773413897281 38.0 38.0 40.0 32.0 40.0 19 37.455761760897715 40.0 38.0 40.0 32.0 40.0 20 37.353474320241695 40.0 38.0 40.0 32.0 40.0 21 37.3485110056107 40.0 38.0 40.0 32.0 40.0 22 37.37742770824342 40.0 38.0 40.0 32.0 40.0 23 37.398791540785496 40.0 38.0 40.0 32.0 40.0 24 37.359948208890806 40.0 38.0 40.0 32.0 40.0 25 37.23413897280967 40.0 38.0 40.0 32.0 40.0 26 36.985757444971945 40.0 38.0 40.0 32.0 40.0 27 36.81268882175227 40.0 38.0 40.0 32.0 40.0 28 36.48252050064739 38.0 38.0 40.0 27.0 40.0 29 36.274492878722484 38.0 38.0 40.0 27.0 40.0 30 35.91799741044454 38.0 38.0 40.0 27.0 40.0 31 35.5558912386707 38.0 38.0 40.0 27.0 40.0 32 35.1124298662063 38.0 32.0 40.0 13.0 40.0 33 34.522874406560206 38.0 32.0 40.0 13.0 40.0 34 33.944756150194216 38.0 32.0 40.0 13.0 40.0 35 33.496331463098834 38.0 32.0 40.0 13.0 40.0 36 32.88260681916271 38.0 27.0 40.0 13.0 40.0 37 32.346568839015966 38.0 27.0 40.0 13.0 40.0 38 31.863185153215365 38.0 27.0 40.0 13.0 40.0 39 31.547475183426844 38.0 27.0 40.0 13.0 40.0 40 31.224859732412604 38.0 27.0 38.0 13.0 40.0 41 30.878506689684937 38.0 27.0 38.0 13.0 40.0 42 30.387570133793698 38.0 27.0 38.0 13.0 40.0 43 30.182347863616744 38.0 27.0 38.0 13.0 40.0 44 29.963314630988346 32.0 13.0 38.0 13.0 40.0 45 29.707596029348295 32.0 13.0 38.0 13.0 40.0 46 29.615666810530858 32.0 13.0 38.0 13.0 40.0 47 29.400517911091928 32.0 13.0 38.0 13.0 40.0 48 29.333189469141132 32.0 13.0 38.0 13.0 40.0 49 29.237807509710834 32.0 13.0 38.0 13.0 40.0 50 29.11286145878291 32.0 13.0 38.0 13.0 40.0 51 28.027621924902892 32.0 13.0 38.0 13.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2201 1 2.0624255952380963 2201 2 1.1369853670634953 2201 3 1.201915922619044 2201 4 1.008010912698417 2201 5 1.0603174603174566 2201 6 0.2724826388888957 2201 7 0.7220362103174622 2201 8 2.0326512896825335 2201 9 1.9499875992063522 2201 10 0.9596788194444485 2201 11 1.4906125992063508 2201 12 1.6378720238095283 2201 13 -1.9302765376984183 2201 14 1.6073474702380892 2201 15 2.021620783730164 2201 16 -2.0069568452380935 2201 17 0.34084201388887436 2201 18 -2.5581473214285637 2201 19 -2.150551835317458 2201 20 2.1197606646825307 2201 21 1.4162946428571388 2201 22 -1.5093749999999986 2201 23 -1.5782180059523796 2201 24 -1.6178199404761813 2201 25 0.7976996527777871 2201 26 3.132669890873011 2201 27 2.0209883432539684 2201 28 1.7810391865079325 2201 29 3.29676959325397 2201 30 3.6971912202381105 2201 31 -0.273518105158729 2201 32 -0.15547495039682957 2201 33 1.753683035714289 2201 34 -1.7766369047619008 2201 35 0.3050533234127002 2201 36 2.8974578373015945 2201 37 1.3279699900793673 2201 38 1.083041914682532 2201 39 2.222495039682542 2201 40 5.829823908730152 2201 41 -0.4849330357142918 2201 42 2.8503162202381027 2201 43 2.820132688492059 2201 44 6.322079613095234 2201 45 7.827132936507933 2201 46 0.7138888888888921 2201 47 1.8212549603174644 2201 48 0.9421812996031775 2201 49 6.923245287698414 2201 50 9.111185515873018 2201 51 6.365097966269836 1101 1 1.0624255952380963 1101 2 1.1369853670634953 1101 3 2.201915922619044 1101 4 2.008010912698417 1101 5 2.0603174603174566 1101 6 2.2724826388888957 1101 7 2.055369543650791 1101 8 0.6993179563491978 1101 9 2.283320932539681 1101 10 2.626345486111113 1101 11 1.157279265873015 1101 12 1.6378720238095283 1101 13 1.9030567956349174 1101 14 1.6073474702380892 1101 15 2.021620783730164 1101 16 2.4930431547619065 1101 17 2.17417534722221 1101 18 1.9418526785714363 1101 19 1.6827814980158777 1101 20 1.4530939980158664 1101 21 2.08296130952381 1101 22 2.323958333333337 1101 23 2.255115327380956 1101 24 1.8821800595238187 1101 25 2.297699652777787 1101 26 2.132669890873011 1101 27 2.354321676587297 1101 28 2.2810391865079325 1101 29 3.29676959325397 1101 30 3.6971912202381105 1101 31 3.5598152281746067 1101 32 4.677858382936499 1101 33 5.253683035714289 1101 34 5.556696428571428 1101 35 5.638386656746036 1101 36 5.8974578373015945 1101 37 2.9946366567460316 1101 38 1.083041914682532 1101 39 3.8891617063492063 1101 40 -0.3368427579365125 1101 41 1.5150669642857117 1101 42 1.8503162202380992 1101 43 -1.6798673115079374 1101 44 -1.6779203869047628 1101 45 -0.8395337301587347 1101 46 3.047222222222228 1101 47 1.1545882936507965 1101 48 0.2755146329365097 1101 49 3.2565786210317427 1101 50 1.1111855158730144 1101 51 1.1984312996031719 2202 1 1.2052827380952422 2202 2 -0.4344432043650741 2202 3 0.4876302083333286 2202 4 0.2937251984127016 2202 5 -0.5111111111111128 2202 6 -0.4418030753968196 2202 7 0.9601314484126959 2202 8 -3.443539186507948 2202 9 -0.24048859126983757 2202 10 0.9120597718253975 2202 11 -2.2236731150793645 2202 12 0.875967261904762 2202 13 2.331628224206348 2202 14 0.4644903273809433 2202 15 -1.4545696924603178 2202 16 1.4454241071428555 2202 17 0.26941344246030496 2202 18 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1102 4 0.5080109126984169 1102 5 0.5603174603174566 1102 6 -1.3525173611111043 1102 7 0.8887028769841265 1102 8 -1.4256820436508022 1102 9 1.1166542658730165 1102 10 -0.9986545138888872 1102 11 0.7406125992063508 1102 12 0.05453869047619264 1102 13 1.4030567956349174 1102 14 2.107347470238089 1102 15 2.438287450396828 1102 16 2.659709821428571 1102 17 1.3408420138888744 1102 18 0.3585193452381006 1102 19 1.349448164682542 1102 20 0.45309399801586636 1102 21 1.2496279761904745 1102 22 2.2406250000000014 1102 23 -0.9532180059523796 1102 24 -0.9928199404761813 1102 25 0.8393663194444514 1102 26 -0.40899677579365346 1102 27 2.1876550099206327 1102 28 2.4477058531745968 1102 29 2.713436259920634 1102 30 3.363857886904775 1102 31 2.0598152281746067 1102 32 -2.9888082837301653 1102 33 1.2120163690476247 1102 34 -0.6099702380952365 1102 35 0.6383866567460359 1102 36 -0.43587549603174125 1102 37 -0.17203000992063266 1102 38 2.041375248015868 1102 39 0.22249503968254203 1102 40 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1211 43 5.510608878968249 1211 44 4.226841517857139 1211 45 3.065228174603167 1211 46 3.856746031746038 1211 47 3.249826388888895 1211 48 -2.7721044146825378 1211 49 -2.2672309027777793 1211 50 5.777852182539682 1211 51 4.198431299603172 1208 1 -0.9375744047619037 1208 2 0.6369853670634953 1208 3 -0.0480840773809561 1208 4 -3.991989087301583 1208 5 -0.18968253968254345 1208 6 0.02248263888889568 1208 7 -0.11129712301587347 1208 8 1.1993179563491978 1208 9 0.3666542658730165 1208 10 -0.6236545138888872 1208 11 0.4906125992063508 1208 12 1.0545386904761926 1208 13 1.4030567956349174 1208 14 0.3573474702380892 1208 15 1.4382874503968281 1208 16 -1.3402901785714292 1208 17 -2.2841579861111256 1208 18 -4.516480654761899 1208 19 -0.900551835317458 1208 20 -0.04690600198413364 1208 21 -0.2503720238095255 1208 22 -1.0093749999999986 1208 23 -2.4532180059523796 1208 24 0.3821800595238187 1208 25 0.7143663194444514 1208 26 -0.9089967757936535 1208 27 -1.9373449900793673 1208 28 1.4477058531745968 1208 29 0.9634362599206341 1208 30 -0.5111421130952252 1208 31 -4.190184771825393 1208 32 -1.9888082837301653 1208 33 0.08701636904762466 1208 34 -2.1099702380952365 1208 35 -3.236613343253964 1208 36 0.5641245039682588 1208 37 3.8279699900793673 1208 38 -0.08362475198413222 1208 39 1.722495039682542 1208 40 0.6631572420634875 1208 41 0.681733630952376 1208 42 -1.608017113095233 1208 43 0.5284660218253947 1208 44 2.0304129464285694 1208 45 2.1187996031745975 1208 46 -0.9111111111111079 1208 47 2.4462549603174644 1208 48 0.8171812996031775 1208 49 1.3399119543650784 1208 50 5.527852182539682 1208 51 2.073431299603172 1209 1 0.06242559523809632 1209 2 -0.6963479662698404 1209 3 1.201915922619044 1209 4 -0.9919890873015831 1209 5 -1.7730158730158792 1209 6 -1.06085069444444 1209 7 0.05536954365079083 1209 8 0.0326512896825335 1209 9 -0.7166790674603192 1209 10 0.29301215277777715 1209 11 0.1572792658730151 1209 12 -1.3621279761904717 1209 13 -0.43027653769841834 1209 14 -1.559319196428575 1209 15 1.3549541170634924 1209 16 0.4930431547619065 1209 17 -0.8258246527777899 1209 18 -3.224813988095235 1209 19 1.6827814980158777 1209 20 -3.3802393353174693 1209 21 1.0829613095238102 1209 22 0.9906250000000014 1209 23 0.9217819940476204 1209 24 0.548846726190483 1209 25 1.6310329861111157 1209 26 1.4660032242063465 1209 27 -0.47901165674603163 1209 28 -0.38562748015873893 1209 29 -2.036563740079366 1209 30 -1.1361421130952252 1209 31 -0.273518105158729 1209 32 1.677858382936499 1209 33 3.253683035714289 1209 34 4.8900297619047635 1209 35 2.9717199900793716 1209 36 -0.7692088293650734 1209 37 1.661303323412703 1209 38 -3.583624751984132 1209 39 3.8891617063492063 1209 40 -3.0035094246031804 1209 41 -2.151599702380956 1209 42 0.016982886904767014 1209 43 -0.6798673115079374 1209 44 -2.5112537202380985 1209 45 -2.6728670634920704 1209 46 1.7138888888888921 1209 47 -7.512078373015868 1209 48 0.9421812996031775 1209 49 -4.910088045634922 1209 50 -4.722147817460318 1209 51 -0.968235367063496 1214 1 -0.18757440476190368 1214 2 -0.11301463293650471 1214 3 0.7019159226190439 1214 4 1.258010912698417 1214 5 0.5603174603174566 1214 6 -0.6025173611111043 1214 7 1.3887028769841265 1214 8 -2.425682043650802 1214 9 -2.3833457341269835 1214 10 0.6263454861111128 1214 11 0.11561259920635081 1214 12 1.3045386904761926 1214 13 0.27805679563491736 1214 14 -1.5176525297619108 1214 15 -2.686712549603172 1214 16 0.15970982142857082 1214 17 -2.0341579861111256 1214 18 0.8585193452381006 1214 19 0.849448164682542 1214 20 0.9530939980158664 1214 21 0.9996279761904745 1214 22 -1.8843749999999986 1214 23 0.5467819940476204 1214 24 -3.1178199404761813 1214 25 -0.5356336805555486 1214 26 1.4660032242063465 1214 27 -2.0623449900793673 1214 28 1.3227058531745968 1214 29 1.213436259920634 1214 30 -0.5111421130952252 1214 31 -1.6901847718253933 1214 32 0.13619171626983473 1214 33 -2.1629836309523753 1214 34 -3.1099702380952365 1214 35 -2.986613343253964 1214 36 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-0.2593749999999986 1215 23 1.1717819940476204 1215 24 -0.24281994047618127 1215 25 0.4643663194444514 1215 26 0.7160032242063465 1215 27 -3.8123449900793673 1215 28 -0.42729414682540323 1215 29 0.5884362599206341 1215 30 -3.886142113095225 1215 31 1.4348152281746067 1215 32 0.13619171626983473 1215 33 -1.9129836309523753 1215 34 0.14002976190476346 1215 35 0.1383866567460359 1215 36 -1.5608754960317412 1215 37 -4.672030009920633 1215 38 3.166375248015868 1215 39 -0.527504960317458 1215 40 1.1631572420634875 1215 41 1.431733630952376 1215 42 -5.358017113095233 1215 43 -3.2215339781746053 1215 44 1.2804129464285694 1215 45 -5.3812003968254025 1215 46 -10.036111111111108 1215 47 -2.3037450396825356 1215 48 -4.9328187003968225 1215 49 -2.1600880456349216 1215 50 -0.9721478174603178 1215 51 -0.30156870039682815 1212 1 2.0624255952380963 1212 2 1.2798425099206412 1212 3 1.3447730654761898 1212 4 1.1508680555555628 1212 5 0.34603174603174125 1212 6 1.7010540674603263 1212 7 1.8172743055555571 1212 8 0.6993179563491978 1212 9 0.4737971230158706 1212 10 2.0549169146825434 1212 11 1.9191840277777814 1212 12 1.7331101190476232 1212 13 -2.0969432043650826 1212 14 0.8930617559523739 1212 15 2.1168588789682588 1212 16 2.5882812500000014 1212 17 1.4122705853174438 1212 18 1.17994791666667 1212 19 1.7780195932539726 1212 20 2.1673797123015817 1212 21 -1.678943452380956 1212 22 0.5620535714285708 1212 23 -2.792503720238095 1212 24 2.3107514880952493 1212 25 1.5357948908730208 1212 26 2.894574652777777 1212 27 3.1162264384920633 1212 28 3.3762772817460274 1212 29 3.6777219742063494 1212 30 -0.06471354166665577 1212 31 0.13124379960317611 1212 32 0.5826202876984041 1212 33 -0.8415550595238059 1212 34 -0.9671130952380906 1212 35 0.0669580853174665 1212 36 -3.007304067460314 1212 37 -5.457744295634917 1212 38 -1.5836247519841322 1212 39 -0.13464781746031562 1212 40 -0.7654141865079396 1212 41 1.7531622023809454 1212 42 1.445554315476194 1212 43 -0.3465339781746053 1212 44 -0.0588727678571459 1212 45 1.6366567460317398 1212 46 2.142460317460319 1212 47 4.821254960317464 1212 48 2.3707527281746046 1212 49 -1.552945188492064 1212 50 2.063566468253967 1212 51 -0.2301401289682552 1213 1 1.2052827380952422 1213 2 0.42269965277778 1213 3 1.3447730654761898 1213 4 0.2937251984127016 1213 5 1.2031746031746025 1213 6 -0.4418030753968196 1213 7 -0.6112971230158735 1213 8 1.556460813492052 1213 9 -1.811917162698414 1213 10 -2.0879402281746025 1213 11 -3.0808159722222186 1213 12 -2.124032738095238 1213 13 2.0459139384920633 1213 14 1.7502046130952351 1213 15 -2.8831411210317412 1213 16 -0.4117187499999986 1213 17 -0.016300843253979735 1213 18 -2.1057663690476147 1213 19 -4.079123263888889 1213 20 -5.832620287698418 1213 21 -1.5360863095238102 1213 22 0.2763392857142861 1213 23 -2.649646577380949 1213 24 -4.260677083333327 1213 25 -3.3213479662698333 1213 26 -2.962568204365084 1213 27 -0.598059275793652 1213 28 -5.195151289682546 1213 29 -2.036563740079366 1213 30 -5.064713541666652 1213 31 0.274100942460322 1213 32 -1.8459511408730194 1213 33 -0.69869791666666 1213 34 0.31860119047619406 1213 35 2.781243799603182 1213 36 1.7069816468254047 1213 37 3.8279699900793673 1213 38 1.4163752480158678 1213 39 0.722495039682542 1213 40 5.6631572420634875 1213 41 -2.6754092261904816 1213 42 3.4455543154761976 1213 43 2.9391803075396794 1213 44 0.22684151785714235 1213 45 5.065228174603167 1213 46 6.571031746031746 1213 47 0.678397817460322 1213 48 7.085038442460323 1213 49 7.304197668650794 1213 50 3.063566468253967 1213 51 1.627002728174599 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 0.0 22 7.0 23 13.0 24 27.0 25 63.0 26 66.0 27 111.0 28 142.0 29 187.0 30 221.0 31 271.0 32 335.0 33 359.0 34 389.0 35 406.0 36 400.0 37 482.0 38 582.0 39 572.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.862694300518136 20.66062176165803 11.830742659758203 19.64594127806563 2 9.926629261976693 25.16184721622788 34.0958135520069 30.815709969788518 3 33.12473025463962 30.2977988778593 25.226586102719033 11.350884764782046 4 17.242123435476913 16.896849374190765 36.75010789814415 29.110919292188175 5 11.178247734138973 28.700906344410875 42.96504100129478 17.155804920155372 6 39.38282261545101 33.103150625809235 10.27190332326284 17.242123435476913 7 14.177816141562365 38.60595597755719 24.082865774708676 23.133362106171774 8 21.709106603366422 25.722917565817866 29.39145446698317 23.176521363832542 9 19.507984462667242 27.319810099266295 23.11178247734139 30.060422960725074 10 14.005179110919292 25.2697453603798 28.91670263271472 31.808372895986185 11 23.025463962019856 24.190763918860593 17.04790677600345 35.735865343116096 12 21.12645662494605 35.00215796288304 26.19766940008632 17.673716012084594 13 23.716012084592144 19.874838152783774 25.722917565817866 30.686232196806213 14 20.198532585239533 24.751834268450583 32.73629693569271 22.313336210617177 15 31.851532153646957 19.810099266292617 26.391886059559777 21.94648252050065 16 13.120414328873546 34.246870953819595 22.248597324126024 30.384117393180837 17 19.572723349158395 31.808372895986185 30.837289598618906 17.781614156236515 18 15.407854984894259 25.93871385412171 41.69184290030212 16.961588260681918 19 14.52309020284851 36.275356063875705 31.398359948208892 17.8031937850669 20 24.126025032369444 23.99654725938714 33.103150625809235 18.774277082434182 21 8.54553301683211 33.18946914113077 36.879585671126456 21.385412170910662 22 12.451445835131636 39.274924471299094 34.268450582649976 14.005179110919292 23 8.847647820457489 32.95209322399655 40.159689253344844 18.04056970220112 24 11.73931808372896 36.922744928787225 40.56970220112214 10.768234786361674 25 13.09883470004316 35.28269313767803 38.56279671989642 13.055675442382391 26 12.084592145015106 34.894259818731115 40.504963314630984 12.516184721622789 27 12.386706948640484 37.28959861890376 40.742339231765214 9.581355200690549 28 10.984031074665515 36.31851532153647 40.138109624514456 12.559343979283558 29 13.120414328873546 35.00215796288304 39.90073370738023 11.976694000863185 30 14.22404489531621 35.527735808331535 40.08202028922944 10.166199007122815 31 15.29995684074234 33.621061717738456 37.28959861890376 13.789382822615451 32 14.93310315062581 33.987915407854985 37.00906344410876 14.069917997410444 33 16.87526974536038 33.987915407854985 35.34743202416919 13.789382822615451 34 17.285282693137678 33.23262839879154 34.311609840310744 15.170479067760034 35 19.054812257229177 32.39102287440656 33.383685800604226 15.170479067760034 36 20.71644367716875 31.59257660768235 31.03150625809236 16.65947345705654 37 19.033232628398792 31.95943029779888 30.686232196806213 18.321104876996117 38 20.953819594302978 29.93094518774277 30.017263703064305 19.097971514889945 39 21.363832542080278 28.463530427276652 29.326715580492014 20.84592145015106 40 22.011221406991798 27.945619335347434 30.75097108329737 19.2921881743634 41 23.608113940440226 26.909797151488995 29.585671126456624 19.896417781614158 42 25.48554164868364 26.003452740612857 29.24039706517048 19.270608545533015 43 24.514458351316357 26.24082865774709 29.326715580492014 19.917997410444542 44 23.457056538627537 27.902460077686662 29.67198964177816 18.96849374190764 45 22.48597324126025 25.183426845058264 30.923608113940443 21.406991799741046 46 23.06862321968062 26.24082865774709 28.91670263271472 21.773845489857575 47 22.572291756581787 26.34872680189901 28.07509710832974 23.00388433318947 48 20.241691842900302 27.384548985757444 28.593008200258957 23.780750971083297 49 21.687526974536038 27.233491583944758 27.837721191195513 23.241260250323695 50 18.53690116529996 26.629261976694004 29.974104445403537 24.859732412602504 51 18.321104876996117 27.082434182132065 26.19766940008632 28.3987915407855 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 0.0 7 0.0 8 0.0 9 0.5 10 1.0 11 2.5 12 4.0 13 2.5 14 1.0 15 2.0 16 3.0 17 7.5 18 12.0 19 14.5 20 17.0 21 15.5 22 14.0 23 22.0 24 30.0 25 37.0 26 82.0 27 120.0 28 155.0 29 190.0 30 222.0 31 254.0 32 332.5 33 411.0 34 514.0 35 617.0 36 655.5 37 694.0 38 670.0 39 646.0 40 500.0 41 354.0 42 294.5 43 235.0 44 223.5 45 212.0 46 189.5 47 167.0 48 157.0 49 147.0 50 144.5 51 142.0 52 127.0 53 112.0 54 88.0 55 64.0 56 53.0 57 42.0 58 34.5 59 27.0 60 25.0 61 23.0 62 20.5 63 18.0 64 15.5 65 13.0 66 10.5 67 8.0 68 6.0 69 4.0 70 3.0 71 2.0 72 1.5 73 1.0 74 1.0 75 1.5 76 2.0 77 1.5 78 1.0 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04315925766076824 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.02157962883038412 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 4634.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.30599913681485 #Duplication Level Percentage of deduplicated Percentage of total 1 88.99028554607006 65.23521795425118 2 5.063291139240507 7.423392317652136 3 2.1195172210774214 4.66119982736297 4 0.8831321754489255 2.589555459646094 5 0.44156608772446276 1.6184721622788087 6 0.5004415660877244 2.20112214069918 7 0.588754783632617 3.0211480362537766 8 0.3826906093612011 2.244281398359948 9 0.20606417427141593 1.3595166163141994 >10 0.8242566970856637 9.6460940871817 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATAC 37 0.7984462667242124 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGA 28 0.6042296072507553 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACC 26 0.561070349589987 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGAT 25 0.539490720759603 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC 23 0.49633146309883475 RNA PCR Primer, Index 45 (95% over 21bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGC 23 0.49633146309883475 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATA 23 0.49633146309883475 No Hit GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAGTACTCTGCGTTGATA 18 0.38843331894691413 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACG 16 0.3452740612861459 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC 16 0.3452740612861459 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATC 15 0.32369443245576174 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAA 15 0.32369443245576174 No Hit CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC 14 0.3021148036253776 RNA PCR Primer, Index 23 (95% over 21bp) GATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG 13 0.2805351747949935 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTAT 13 0.2805351747949935 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATC 13 0.2805351747949935 No Hit ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTT 13 0.2805351747949935 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTCGAAGCAGTGGTATCAACG 12 0.2589555459646094 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC 11 0.2373759171342253 No Hit ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGT 11 0.2373759171342253 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAGTACTCTGCGTT 11 0.2373759171342253 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAA 11 0.2373759171342253 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGT 10 0.21579628830384118 No Hit CCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTT 10 0.21579628830384118 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCA 10 0.21579628830384118 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTA 10 0.21579628830384118 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAGTACTCTGCGTTG 10 0.21579628830384118 No Hit CAGTGGTATCAACGCAGAGTACTTTTTGCAAGCAGTGGTATCAACGCAGAG 10 0.21579628830384118 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAGTACTCTGCGTTGATACCA 9 0.19421665947345707 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTAT 9 0.19421665947345707 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC 9 0.19421665947345707 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCA 9 0.19421665947345707 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGT 9 0.19421665947345707 No Hit GCGTTGATACCACTGCTTCAAAAAAAAGTACTCTGCGTTGATACCACTGCT 9 0.19421665947345707 No Hit GCGTTGATACCACTGCTTGCAAAAAGTACTCTGCGTTGATACCACTGCTTG 9 0.19421665947345707 No Hit GTGGTATCAACGCAGAGTACTTTTTGCAAGCAGTGGTATCAACGCAGAGTA 8 0.17263703064307295 No Hit ACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAGAGTA 8 0.17263703064307295 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGG 8 0.17263703064307295 No Hit TACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT 8 0.17263703064307295 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACG 8 0.17263703064307295 No Hit TACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG 8 0.17263703064307295 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAA 8 0.17263703064307295 No Hit ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTG 8 0.17263703064307295 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTGCGAAGCAGTG 8 0.17263703064307295 No Hit GGTATCAACGCAGAGTACTTTTTGCAAGCAGTGGTATCAACGCAGAGTACT 8 0.17263703064307295 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTCGAAGCAGTGGTATCAACGC 8 0.17263703064307295 No Hit CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC 8 0.17263703064307295 RNA PCR Primer, Index 27 (95% over 21bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGTAT 8 0.17263703064307295 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCA 7 0.1510574018126888 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGC 7 0.1510574018126888 No Hit TACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT 7 0.1510574018126888 No Hit CCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTTC 7 0.1510574018126888 No Hit GATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT 7 0.1510574018126888 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGT 7 0.1510574018126888 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAGTACTCTGCGTTGATA 7 0.1510574018126888 No Hit AAAAAAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAA 7 0.1510574018126888 No Hit GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAGTACTCTGCGTTGATACC 7 0.1510574018126888 No Hit GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAGTACTCTGCGTTGAT 7 0.1510574018126888 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAA 7 0.1510574018126888 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTAC 7 0.1510574018126888 No Hit GTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCA 7 0.1510574018126888 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAGTACTCTGCGTTGATACC 7 0.1510574018126888 No Hit GATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCA 7 0.1510574018126888 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCA 7 0.1510574018126888 No Hit CCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT 7 0.1510574018126888 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCA 7 0.1510574018126888 No Hit ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCG 7 0.1510574018126888 No Hit TGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCAC 7 0.1510574018126888 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTA 6 0.1294777729823047 No Hit ATACCACTGCTTGCAAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAA 6 0.1294777729823047 No Hit GTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGC 6 0.1294777729823047 No Hit GTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTTT 6 0.1294777729823047 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTCGAAGCAGTGGTATCAACGC 6 0.1294777729823047 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACG 6 0.1294777729823047 No Hit ATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGC 6 0.1294777729823047 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCAAGCAGTGGTA 6 0.1294777729823047 No Hit GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAGTACTCTGCGTTGATAC 6 0.1294777729823047 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTCGAAGCAGTGGTATCA 6 0.1294777729823047 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATA 6 0.1294777729823047 No Hit ACGCAGAGTACTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAGAGTACT 6 0.1294777729823047 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATC 6 0.1294777729823047 No Hit GTATCAACGCAGAGTACTTTTTGCAAGCAGTGGTATCAACGCAGAGTACTT 6 0.1294777729823047 No Hit GCGTTGATACCACTGCTTGCAAAAAGTACTCTGCGTTGATACCACTGCTTC 6 0.1294777729823047 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.1294777729823047 No Hit GTATCAACGCAGAGTACTTTTTTTTGAAGCAGTGGTATCAACGCAGAGTAC 6 0.1294777729823047 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTGCGAAGCAGTGG 5 0.10789814415192059 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTGAAGCAGTGGTATCAACGCAGAG 5 0.10789814415192059 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTGCAAGCAGTGGTATCAACGCA 5 0.10789814415192059 No Hit GTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCAC 5 0.10789814415192059 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTCGAAGCAGTGGTATCAAC 5 0.10789814415192059 No Hit CTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAGTACTCTGCGT 5 0.10789814415192059 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAG 5 0.10789814415192059 No Hit TGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT 5 0.10789814415192059 No Hit ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGC 5 0.10789814415192059 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC 5 0.10789814415192059 No Hit TACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGC 5 0.10789814415192059 No Hit ATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT 5 0.10789814415192059 No Hit CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC 5 0.10789814415192059 No Hit GTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATAC 5 0.10789814415192059 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAGTACTCTGCG 5 0.10789814415192059 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.04315925766076824 21 0.0 0.0 0.06473888649115235 22 0.0 0.0 0.06473888649115235 23 0.0 0.0 0.06473888649115235 24 0.0 0.0 0.08631851532153648 25 0.0 0.0 0.08631851532153648 26 0.0 0.0 0.10789814415192059 27 0.0 0.0 0.1294777729823047 28 0.0 0.0 0.1294777729823047 29 0.0 0.0 0.1510574018126888 30 0.0 0.0 0.21579628830384118 31 0.0 0.0 0.41001294777729824 32 0.0 0.0 0.8847647820457488 33 0.0 0.0 1.1652999568407423 34 0.0 0.0 1.6832110487699612 35 0.0 0.0 1.9637462235649548 36 0.0 0.0 2.352179542511869 37 0.0 0.0 3.1722054380664653 38 0.0 0.0 4.03539059128183 39 0.0 0.0 4.51014242555028 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCGCAA 105 2.1660628E-5 17.142857 17 TCGCAAA 105 2.1660628E-5 17.142857 18 CTTCGCA 105 2.1660628E-5 17.142857 16 GCTTCGC 105 2.1660628E-5 17.142857 15 CGCAAAA 105 2.1660628E-5 17.142857 19 TGCTTCG 140 7.21393E-7 16.071428 14 CAAAAAA 115 4.7376736E-5 15.652173 21 CTGCTTC 150 1.4864418E-6 15.0 13 GCAAAAA 120 6.824552E-5 14.999999 20 ACTGCTT 165 4.018215E-6 13.636363 12 CACTGCT 165 6.748246E-5 12.272727 11 GTTGATA 185 1.3162646E-5 12.162163 3 CGTTGAT 185 1.3162646E-5 12.162163 2 CCACTGC 170 8.933141E-5 11.911764 10 ATACCAC 170 8.933141E-5 11.911764 7 TGGTATC 135 0.0030672555 11.666666 4 ACCACTG 175 1.1723521E-4 11.571429 9 TTGATAC 195 2.2657056E-5 11.538462 4 GCGTTGA 195 2.2657056E-5 11.538462 1 GATACCA 180 1.5260378E-4 11.250001 6 >>END_MODULE