FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_2.34100000006232.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_2.34100000006232.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4634
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATAC370.7984462667242124No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGA280.6042296072507553No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACC260.561070349589987No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGAT250.539490720759603No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC230.49633146309883475RNA PCR Primer, Index 45 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGC230.49633146309883475No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATA230.49633146309883475No Hit
GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAGTACTCTGCGTTGATA180.38843331894691413No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACG160.3452740612861459No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC160.3452740612861459No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATC150.32369443245576174No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAA150.32369443245576174No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC140.3021148036253776RNA PCR Primer, Index 23 (95% over 21bp)
GATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG130.2805351747949935No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTAT130.2805351747949935No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATC130.2805351747949935No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTT130.2805351747949935No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTCGAAGCAGTGGTATCAACG120.2589555459646094No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC110.2373759171342253No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGT110.2373759171342253No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAGTACTCTGCGTT110.2373759171342253No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAA110.2373759171342253No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGT100.21579628830384118No Hit
CCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTT100.21579628830384118No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCA100.21579628830384118No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTA100.21579628830384118No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAGTACTCTGCGTTG100.21579628830384118No Hit
CAGTGGTATCAACGCAGAGTACTTTTTGCAAGCAGTGGTATCAACGCAGAG100.21579628830384118No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAGTACTCTGCGTTGATACCA90.19421665947345707No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTAT90.19421665947345707No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC90.19421665947345707No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCA90.19421665947345707No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGT90.19421665947345707No Hit
GCGTTGATACCACTGCTTCAAAAAAAAGTACTCTGCGTTGATACCACTGCT90.19421665947345707No Hit
GCGTTGATACCACTGCTTGCAAAAAGTACTCTGCGTTGATACCACTGCTTG90.19421665947345707No Hit
GTGGTATCAACGCAGAGTACTTTTTGCAAGCAGTGGTATCAACGCAGAGTA80.17263703064307295No Hit
ACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAGAGTA80.17263703064307295No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGG80.17263703064307295No Hit
TACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT80.17263703064307295No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACG80.17263703064307295No Hit
TACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG80.17263703064307295No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAA80.17263703064307295No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTG80.17263703064307295No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTGCGAAGCAGTG80.17263703064307295No Hit
GGTATCAACGCAGAGTACTTTTTGCAAGCAGTGGTATCAACGCAGAGTACT80.17263703064307295No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTCGAAGCAGTGGTATCAACGC80.17263703064307295No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC80.17263703064307295RNA PCR Primer, Index 27 (95% over 21bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGTAT80.17263703064307295No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCA70.1510574018126888No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGC70.1510574018126888No Hit
TACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT70.1510574018126888No Hit
CCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTTC70.1510574018126888No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT70.1510574018126888No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGT70.1510574018126888No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAGTACTCTGCGTTGATA70.1510574018126888No Hit
AAAAAAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAA70.1510574018126888No Hit
GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAGTACTCTGCGTTGATACC70.1510574018126888No Hit
GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAGTACTCTGCGTTGAT70.1510574018126888No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAA70.1510574018126888No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTAC70.1510574018126888No Hit
GTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCA70.1510574018126888No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAGTACTCTGCGTTGATACC70.1510574018126888No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCA70.1510574018126888No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCA70.1510574018126888No Hit
CCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT70.1510574018126888No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCA70.1510574018126888No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCG70.1510574018126888No Hit
TGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCAC70.1510574018126888No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTA60.1294777729823047No Hit
ATACCACTGCTTGCAAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAA60.1294777729823047No Hit
GTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGC60.1294777729823047No Hit
GTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTTT60.1294777729823047No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTCGAAGCAGTGGTATCAACGC60.1294777729823047No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACG60.1294777729823047No Hit
ATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGC60.1294777729823047No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCAAGCAGTGGTA60.1294777729823047No Hit
GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAGTACTCTGCGTTGATAC60.1294777729823047No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTCGAAGCAGTGGTATCA60.1294777729823047No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATA60.1294777729823047No Hit
ACGCAGAGTACTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAGAGTACT60.1294777729823047No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATC60.1294777729823047No Hit
GTATCAACGCAGAGTACTTTTTGCAAGCAGTGGTATCAACGCAGAGTACTT60.1294777729823047No Hit
GCGTTGATACCACTGCTTGCAAAAAGTACTCTGCGTTGATACCACTGCTTC60.1294777729823047No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.1294777729823047No Hit
GTATCAACGCAGAGTACTTTTTTTTGAAGCAGTGGTATCAACGCAGAGTAC60.1294777729823047No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTGCGAAGCAGTGG50.10789814415192059No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTGAAGCAGTGGTATCAACGCAGAG50.10789814415192059No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTGCAAGCAGTGGTATCAACGCA50.10789814415192059No Hit
GTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCAC50.10789814415192059No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTCGAAGCAGTGGTATCAAC50.10789814415192059No Hit
CTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAGTACTCTGCGT50.10789814415192059No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAG50.10789814415192059No Hit
TGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT50.10789814415192059No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGC50.10789814415192059No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC50.10789814415192059No Hit
TACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGC50.10789814415192059No Hit
ATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT50.10789814415192059No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC50.10789814415192059No Hit
GTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATAC50.10789814415192059No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAGTACTCTGCG50.10789814415192059No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCAA1052.1660628E-517.14285717
TCGCAAA1052.1660628E-517.14285718
CTTCGCA1052.1660628E-517.14285716
GCTTCGC1052.1660628E-517.14285715
CGCAAAA1052.1660628E-517.14285719
TGCTTCG1407.21393E-716.07142814
CAAAAAA1154.7376736E-515.65217321
CTGCTTC1501.4864418E-615.013
GCAAAAA1206.824552E-514.99999920
ACTGCTT1654.018215E-613.63636312
CACTGCT1656.748246E-512.27272711
GTTGATA1851.3162646E-512.1621633
CGTTGAT1851.3162646E-512.1621632
CCACTGC1708.933141E-511.91176410
ATACCAC1708.933141E-511.9117647
TGGTATC1350.003067255511.6666664
ACCACTG1751.1723521E-411.5714299
TTGATAC1952.2657056E-511.5384624
GCGTTGA1952.2657056E-511.5384621
GATACCA1801.5260378E-411.2500016
Produced by FastQC (version 0.11.2)