FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_19.3410000000634e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_19.3410000000634e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585246
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACT601.546141E-1037.4989323
CGACGCG250.002105676836.0020517
CGCGCAA250.002106560235.99897420
ACGCGCA250.002106560235.99897419
ACGCCTA453.4886245E-529.99914621
ACGGGTC406.1604445E-428.12660216
TATGCCG903.947207E-1027.49921643
TACATCG450.001226038724.9992895
TCGTATG1001.3424142E-924.74929440
ACGAGAC1001.3424142E-924.74929422
TGCCGTC1052.3610482E-923.57075945
TATAAGG602.4631253E-422.5032062
ATATAGG500.002261419822.5032022
CCTAATC602.4659763E-422.49936124
CGCCTAA602.4659763E-422.49936122
TCTCGTA1104.0436134E-922.4993638
GCAATCG500.002263666822.4993623
TATCTCG754.598686E-520.99940336
CGGACAC654.2356204E-420.7686395
CTCGTAT1201.1028533E-820.62441339