FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_18.34100000006331.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_18.34100000006331.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500302
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGA250.0021066435.997682
GGTATCA4350.034.6806681
TCGCGGG300.005126043730.0220721
TATGCCG453.488479E-529.99806843
GCGGGAT450.001226046824.998393
GGGATCG450.001226046824.998395
GCCCACG551.365438E-424.5487818
CCCACGA751.6954018E-623.99845519
GTATCAA9300.023.2428931
GATCCTA602.465883E-422.4985531
TGCCGTC654.2354641E-420.76789345
TGTACTA550.00393494620.4532285
ATCGATT550.00393494620.4532288
TACCGGG550.00393494620.4532282
GTACGGA706.9414807E-419.2999021
ACGGGGA1656.002665E-1119.09349616
CATCTCC18600.018.99071345
TCTCCGA4750.018.94614845
CTGTTAA600.0064779118.7637961
ACGGGTC600.006500389418.7525416
Produced by FastQC (version 0.11.2)