FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_17.34100000006324.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_17.34100000006324.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212706
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA35511.669440448318336No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA35471.6675599183850007No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG31091.461641890684795No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATAC29481.3859505608680525No Hit
GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCG29351.3798388385847131No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATAC26101.227045781501227No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATACA25461.1969573025678637No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACA22891.076133254351076No Hit
TATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGAG22291.047925305351048No Hit
GATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAG22151.0413434505843746No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG18220.85658138463419No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAACTGTCTCTTATA18050.8485891324175152No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG16510.7761887299841095No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATA15990.7517418408507517No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATA15750.7404586612507404No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA15380.7230637593673898No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAACTGTCTCTTATAC14660.6892142205673558No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATA14310.6727595836506728No Hit
TATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCGA13700.6440815021673107No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13390.6295073951839629No Hit
TGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGA13280.624335937867291No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATAC13230.621985275450622No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11790.5542861978505542No Hit
TGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC11590.5448835481838782No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11410.5364211634838698No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAACTGTCTCTTAT10740.5049222871005049No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA10480.49269884253382606No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCC10350.4865871202504866No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAACTGTCTCTTA10260.4823559279004823No Hit
TTTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTA9770.459319436217126No Hit
TTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTAT9600.4513271840004513No Hit
ATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA8990.4226491025170893No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAACTGTCTCTTAT8410.3953814184837287No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAACTGTCTCTTATA8230.3869190337837202No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCTGTCTCTTATACACATCTCCGA7740.36388254210036386No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTAT7710.3624721446503625No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA7580.35636042236702303No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCCG7240.3403759179336737No Hit
TTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATA7170.3370849905503371No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG7170.3370849905503371No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAACTGTCTCTTAT7000.3290927383336624No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6990.3286226058503286No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAACTGTCTCTT6760.31780955873365113No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACCTGTCTCTTATACACATCTC6450.30323545175030325No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA6320.2971237294669638No Hit
TTTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTAT6250.29383280208362716No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAACTGTCTCTTAT6160.2896016097336229No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6140.2886613447669553No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACTGTCTCTTATACACATCTCC6060.2849002849002849No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC6040.2839600199336173No Hit
TTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCC6000.2820794900002821No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAACTGTCTCTTATA5990.28160935751694827No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5850.27502750275027504No Hit
TTTTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTT5830.27408723778360744No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACCTGTCTCTTATACACATCTCC5580.2623339257002623No Hit
TTTTTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACTGTCTCTTATA5560.26139366073359477No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT5420.2548118059669215No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC5410.25434167348358766No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATCTC5360.2519910110669187No Hit
GTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTC5280.24822995120024824No Hit
GTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCC5200.2444688913335778No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATCT5190.24399875885024402No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATCT4950.23271557925023273No Hit
TTTTTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTAT4910.23083504931689752No Hit
GATACCACCCCGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTC4730.222372664616889No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATC4730.222372664616889No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAACTGTCTCTTATACA4470.21014922005021014No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATACAC4440.2087388226002087No Hit
TTTTCTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATA4410.20732842515020733No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC4410.20732842515020733No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCC4360.20497776273353832No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT4340.2040374977668707No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC4340.2040374977668707No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAACTGTCTCTTA4320.20309723280020311No Hit
ATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAG4210.19792577548353127No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATCTGTCTCTTATACAC4070.19134392071685802No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCCTGTCTCTTATACACATCTCC4050.19040365575019041No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAGAAAACTGTCTCTTATA4050.19040365575019041No Hit
TGATACCACCCCGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCT3990.18758286085018758No Hit
TTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTA3920.18429193346685097No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC3910.18382180098351714No Hit
GTATCAACGCAGAGTACGGGGTGGTATCTGTCTCTTATACACATCTCCGAG3850.18100100608351433No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAACTGTCTCTTA3800.17865034366684532No Hit
GATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCC3790.17818021118351152No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAGAAAACTGTCTCTTATAC3770.17723994621684389No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAACTGTCTCTTATACAC3750.1762996812501763No Hit
TTTTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTA3750.1762996812501763No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAACTGTCTCTTATAC3720.17488928380017488No Hit
TTTTCTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATAC3690.1734788863501735No Hit
TTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCG3600.16924769400016923No Hit
TTTTTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTTATACA3510.16501650165016502No Hit
GTGGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTC3450.1621957067501622No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAGAAAAACTGTCTCTTAT3360.15796451440015796No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACTGTCTCTTATACACATCTCCG3250.15279305708348612No Hit
GTGGTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCT3130.1471514672834805No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCCGA3120.14668133480014667No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAACTGTCTCTTATACA3110.1462112023168129No Hit
GATACCACCCCGTACTCTGCGTTGATCTGTCTCTTATACACATCTCCGAGC3090.1452709373501453No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAACTGTCTCTTATA3090.1452709373501453No Hit
TTTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTT3070.14433067238347766No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAACTGTCTCTTA2990.14056961251680725No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAACTGTCTCTT2980.14009948003347342No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAACTGTCTCT2970.13962934755013964No Hit
TTTTTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCTTATACAC2970.13962934755013964No Hit
TTTTTTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTA2950.13868908258347204No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAACTGTCTCTTAT2930.1377488176168044No Hit
ATACCACTGCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGA2920.1372786851334706No Hit
GATACCACTGCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCG2880.1353981552001354No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATCTGTCTCTTATACA2880.1353981552001354No Hit
TTTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATCTGTCTCTTATA2820.1325773603001326No Hit
TTTTTTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACTGTCTCTTAT2810.13210722781679876No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCCTGTCTCTTATACACATCTCCG2800.131637095333465No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAGAAAAACTGTCTCTTATA2790.13116696285013116No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2770.13022669788346355No Hit
TTTTTCTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTAT2640.12411497560012412No Hit
TGATACCACCCCGTACTCTGCGTTGATCTGTCTCTTATACACATCTCCGAG2640.12411497560012412No Hit
TTTTTCTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATA2630.1236448431167903No Hit
TTTTTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCT2620.12317471063345652No Hit
TTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATCTGTCTCTTATAC2620.12317471063345652No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACTGTCTCTTATACACATCTC2550.11988378325011988No Hit
TTTTTGCGTTGATACCACCCGTACTCTGCGTTGATACCCTGTCTCTTATAC2550.11988378325011988No Hit
GGTATCAACGCAGAGTACGGGTGGTATCCTGTCTCTTATACACATCTCCGA2540.11941365076678608No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAACTGTCTCTTATAC2510.11800325331678468No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACCTGTCTCTTATACACATC2490.11706298835011707No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA2490.11706298835011707No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACCTGTCTCTTATACACATCT2480.11659285586678325No Hit
TTTTTTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTTATAC2470.11612272338344946No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA2460.11565259090011565No Hit
GTTGATACCACCCCGTACTCTGCGTTGATCTGTCTCTTATACACATCTCCG2450.11518245841678186No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAACTGTCTCTTATAC2430.11424219345011424No Hit
GATACCACCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA2390.11236166351677904No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2360.11095126606677762No Hit
GTATCAACGCAGAGTACGGGTGGTATCCTGTCTCTTATACACATCTCCGAG2350.11048113358344382No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACCTGTCTCTTATACACATCT2340.11001100110011No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACAT2290.10766033868344099No Hit
TTTTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCT2290.10766033868344099No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACAT2290.10766033868344099No Hit
ACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC2290.10766033868344099No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATC2270.10672007371677339No Hit
GTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCT2260.10624994123343957No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATC2240.10530967626677197No Hit
TGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTC2220.10436941130010435No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAACTGTCTCT2210.10389927881677057No Hit
TTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTC2200.10342914633343675No Hit
TTTTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATCTGTCTCTTAT2190.10295901385010296No Hit
GATACCACCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAG2190.10295901385010296No Hit
TTTTTTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCTTATACA2180.10248888136676916No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC650.041.54736345
ATGCCGT650.041.53759444
TTCTGTG250.002103474535.9992453
GTATGCC801.8189894E-1233.74929442
ATCTCGT801.8189894E-1233.74929437
TCCTATC801.8189894E-1233.74929433
TATGCCG801.8189894E-1233.74929443
TATCTCG801.8189894E-1233.74929436
CGTATGC801.8189894E-1233.74929441
TCGTATG801.8189894E-1233.74929440
CTAGATC801.8189894E-1233.74929428
AGATCCT801.8189894E-1233.74929430
TAGATCC801.8189894E-1233.74929429
GAAAAGT352.8014247E-432.1648671
ATCCTAT855.456968E-1231.76404232
TCTCGTA855.456968E-1231.76404238
GATCCTA855.456968E-1231.76404231
ACTAGAT855.456968E-1231.76404227
CGAGACT855.456968E-1231.76404223
CTCGTAT855.456968E-1231.76404239
Produced by FastQC (version 0.11.2)