H23NVBCXX l02n01 24h_15.3410000000630a.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23NVBCXX l02n01 24h_15.3410000000630a.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2323
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC	60	2.582866982350409	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC	38	1.6358157554885924	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC	29	1.248385708136031	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC	25	1.076194575979337	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC	21	0.9040034438226432	RNA PCR Primer, Index 39 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC	14	0.6026689625484287	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC	13	0.5596211795092553	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC	10	0.4304778303917348	RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC	9	0.38743004735256137	No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC	8	0.34438226431338786	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC	7	0.30133448127421436	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC	6	0.2582866982350409	No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC	6	0.2582866982350409	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC	6	0.2582866982350409	TruSeq Adapter, Index 12 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGT	6	0.2582866982350409	No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC	5	0.2152389151958674	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC	4	0.17219113215669393	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT	4	0.17219113215669393	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC	4	0.17219113215669393	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG	3	0.12914334911752046	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	75	0.0	42.0	45
TATCTCG	30	8.240019E-5	37.499996	36
CGAGACT	60	4.5474735E-11	37.499996	23
ATCTCCG	85	0.0	37.058823	10
CATCTCC	85	0.0	37.058823	9
ATGCCGT	85	0.0	37.058823	44
TCTCCGA	85	0.0	37.058823	11
TCCGAGC	85	0.0	37.058823	13
CTCCGAG	85	0.0	37.058823	12
CAATCTC	25	0.0017003631	36.0	35
TATACAC	95	0.0	35.526318	3
TACACAT	95	0.0	35.526318	5
ACACATC	95	0.0	35.526318	6
ATACACA	95	0.0	35.526318	4
AGCCCAC	90	0.0	35.000004	17
ATCTCGT	90	0.0	35.000004	37
CACATCT	90	0.0	35.000004	7
GCCCACG	90	0.0	35.000004	18
CCACGAG	90	0.0	35.000004	20
TATGCCG	90	0.0	35.000004	43

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content