H23NVBCXX l02n01 24h_13.341000000062ef.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23NVBCXX l02n01 24h_13.341000000062ef.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3772
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC	184	4.878048780487805	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC	73	1.9353128313891834	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC	65	1.7232237539766702	RNA PCR Primer, Index 39 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC	54	1.4316012725344645	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC	48	1.2725344644750796	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC	32	0.8483563096500532	RNA PCR Primer, Index 45 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT	23	0.6097560975609756	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC	18	0.47720042417815484	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGT	16	0.4241781548250266	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC	9	0.23860021208907742	RNA PCR Primer, Index 23 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC	8	0.2120890774125133	RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC	6	0.15906680805938495	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGT	6	0.15906680805938495	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC	6	0.15906680805938495	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC	5	0.1325556733828208	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC	5	0.1325556733828208	TruSeq Adapter, Index 12 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT	5	0.1325556733828208	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4	0.10604453870625664	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC	4	0.10604453870625664	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC	4	0.10604453870625664	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCG	4	0.10604453870625664	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4	0.10604453870625664	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4	0.10604453870625664	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC	4	0.10604453870625664	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCTC	20	6.171144E-4	45.000004	35
CGAGACG	20	6.171144E-4	45.000004	23
TGCCGTC	50	1.0913936E-11	45.0	45
ATGCCGT	45	2.1100277E-10	45.0	44
CGAGACT	30	1.6419108E-6	44.999996	23
AGCCCAC	55	2.910383E-11	40.909092	17
ATCTCGT	55	2.910383E-11	40.909092	37
ATCTCCG	55	2.910383E-11	40.909092	10
GCCCACG	55	2.910383E-11	40.909092	18
CCACGAG	55	2.910383E-11	40.909092	20
GAGCCCA	55	2.910383E-11	40.909092	16
CACGAGA	55	2.910383E-11	40.909092	21
CCCACGA	55	2.910383E-11	40.909092	19
ACGAGAC	55	2.910383E-11	40.909092	22
CGAGCCC	55	2.910383E-11	40.909092	15
CCGAGCC	55	2.910383E-11	40.909092	14
TCTCCGA	55	2.910383E-11	40.909092	11
TCCGAGC	55	2.910383E-11	40.909092	13
CTCCGAG	55	2.910383E-11	40.909092	12
GTATGCC	50	5.9480953E-10	40.5	42

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content