FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_12.341000000062d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_12.341000000062d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583719
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1300.036.34532545
ATGCCGT1550.030.48317344
CCACGAG1650.029.99931720
TATGCCG1600.029.53057343
CCCACGA1700.029.11698319
AATCTCG1300.027.69167536
CTCGTAT1800.027.49937239
GTATCAA9750.027.2418161
TCGTATG1950.025.38403740
GCAATAG450.00122293625.0101411
GTTCCGT450.001225994424.999436
CGTATGC1950.024.23021741
CGAGACT1950.024.23021723
GTACTAC952.1018423E-823.6938191
GCCCACG2100.023.5708918
CACGAGA2200.022.49948721
TCTCGTA2200.022.49948738
CGAGCCC2250.021.99949815
ACGAGAC2150.021.97624222
CCGAGCC2350.021.06334914