FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_11.341000000062c5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_11.341000000062c5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491278
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC6040.12294464641201112TruSeq Adapter, Index 12 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACT1400.036.96361523
TATGCCG1500.035.99934843
AATCTCG1450.034.1373136
TGCCGTC1650.032.73334545
GCCCACG1800.031.24943418
TCTCGTA1850.030.40485638
CTCGTAT1900.029.60472539
CCACGAG1900.029.60472520
CCCACGA2000.029.2494719
CGTATGC1950.027.69180741
TCGTATG1950.027.69180740
ATGCCGT1950.027.69180744
AGACTAC2000.026.99951225
GGTATCA3350.026.8816011
ACGAGAC2100.026.7852322
ATCTCGT2150.026.16231537
CGAGCCC2200.025.56771915
GAGACTA2050.025.24344624
CACGAGA2250.024.99954821
TGCAATC1850.024.32388533
Produced by FastQC (version 0.11.2)