FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_10.341000000062b8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_10.341000000062b8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418540
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC5230.12495818798681128TruSeq Adapter, Index 12 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG606.4137566E-933.74901243
GAATATA300.00513228730.0134661
CTAACTA406.160592E-428.124184
CAGTCCG507.1491304E-526.9992129
CGAGCCC801.0507392E-725.31176215
GCCCACG851.888202E-723.82283418
GTATCAA5200.023.3758741
TCTCGTA1056.0863385E-821.42794638
AATCTCG1056.0863385E-821.42794636
GACCATG955.515958E-721.3151687
CGAGACT754.5946897E-520.99938823
CATCTCC12150.020.74013545
AGTCCGA550.003933085620.45394910
CGAATGT550.003933085620.45394912
TGCCGTC1009.029227E-720.24940945
GCAATCT807.5500284E-519.68692634
CGGGGTG706.974687E-419.28515217
CCCTAGA600.00648690918.7584151
GGTATCA2400.018.7584151
CTACTTA600.006504839318.74945313
Produced by FastQC (version 0.11.2)