##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 18h_95.341000000054c7.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2288 Sequences flagged as poor quality 0 Sequence length 51 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.72027972027972 38.0 32.0 38.0 32.0 38.0 2 35.19973776223776 38.0 32.0 38.0 32.0 38.0 3 35.19624125874126 38.0 32.0 38.0 32.0 38.0 4 35.24562937062937 38.0 32.0 38.0 32.0 38.0 5 35.37587412587413 38.0 32.0 38.0 32.0 38.0 6 37.00743006993007 38.0 38.0 40.0 32.0 40.0 7 36.70716783216783 38.0 38.0 40.0 32.0 40.0 8 36.65865384615385 38.0 38.0 40.0 32.0 40.0 9 36.6368006993007 38.0 38.0 40.0 32.0 40.0 10 36.52447552447553 38.0 38.0 40.0 32.0 40.0 11 36.62325174825175 38.0 38.0 40.0 32.0 40.0 12 36.35489510489511 38.0 38.0 40.0 32.0 40.0 13 36.43968531468531 38.0 38.0 40.0 32.0 40.0 14 36.25524475524475 38.0 38.0 40.0 32.0 40.0 15 36.27403846153846 38.0 38.0 40.0 32.0 40.0 16 36.34090909090909 38.0 38.0 40.0 32.0 40.0 17 36.08697552447553 38.0 38.0 40.0 27.0 40.0 18 36.05419580419581 38.0 38.0 40.0 27.0 40.0 19 36.125 38.0 38.0 40.0 32.0 40.0 20 36.21896853146853 38.0 38.0 40.0 27.0 40.0 21 36.29326923076923 38.0 38.0 40.0 32.0 40.0 22 36.50437062937063 38.0 38.0 40.0 32.0 40.0 23 36.27403846153846 38.0 38.0 40.0 32.0 40.0 24 36.14117132867133 38.0 38.0 40.0 32.0 40.0 25 36.27928321678322 38.0 38.0 40.0 32.0 40.0 26 36.1993006993007 38.0 38.0 40.0 32.0 40.0 27 36.11625874125874 38.0 38.0 40.0 27.0 40.0 28 36.01573426573427 38.0 38.0 40.0 27.0 40.0 29 36.01223776223776 38.0 38.0 40.0 27.0 40.0 30 35.91302447552447 38.0 38.0 40.0 27.0 40.0 31 35.93487762237762 38.0 38.0 40.0 27.0 40.0 32 36.05638111888112 38.0 38.0 40.0 27.0 40.0 33 36.18138111888112 38.0 38.0 40.0 27.0 40.0 34 36.20367132867133 38.0 38.0 40.0 27.0 40.0 35 36.12587412587413 38.0 38.0 40.0 32.0 40.0 36 36.01223776223776 38.0 38.0 40.0 27.0 40.0 37 35.8125 38.0 38.0 40.0 27.0 40.0 38 35.91477272727273 38.0 38.0 40.0 27.0 40.0 39 35.90777972027972 38.0 38.0 40.0 27.0 40.0 40 35.76354895104895 38.0 38.0 40.0 27.0 40.0 41 35.84702797202797 38.0 38.0 40.0 27.0 40.0 42 36.05463286713287 38.0 38.0 40.0 27.0 40.0 43 35.99213286713287 38.0 38.0 40.0 27.0 40.0 44 35.78671328671329 38.0 38.0 40.0 27.0 40.0 45 35.95454545454545 38.0 38.0 40.0 27.0 40.0 46 35.61145104895105 38.0 38.0 40.0 27.0 40.0 47 35.4881993006993 38.0 38.0 40.0 27.0 40.0 48 35.56512237762238 38.0 38.0 40.0 27.0 40.0 49 35.37456293706294 38.0 32.0 40.0 27.0 40.0 50 35.39729020979021 38.0 38.0 40.0 27.0 40.0 51 34.82473776223776 38.0 32.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.085185185185196 1101 2 0.5473544973545046 1101 3 0.5777777777777757 1101 4 0.7510582010581999 1101 5 3.203439153439163 1101 6 -0.1579365079365047 1101 7 2.7010582010582027 1101 8 1.532539682539685 1101 9 1.867724867724867 1101 10 1.6174603174603206 1101 11 2.93280423280423 1101 12 2.173280423280417 1101 13 -0.3007936507936506 1101 14 3.608201058201061 1101 15 3.5174603174603263 1101 16 3.5759259259259295 1101 17 1.8891534391534321 1101 18 -0.47989417989418826 1101 19 0.30158730158730407 1101 20 -0.49629629629628624 1101 21 0.25634920634921343 1101 22 1.6119047619047748 1101 23 2.3047619047618966 1101 24 3.781481481481485 1101 25 3.7862433862433917 1101 26 2.215343915343915 1101 27 3.69841269841271 1101 28 2.4330687830687836 1101 29 3.034656084656085 1101 30 -0.47566137566137456 1101 31 -0.20793650793650897 1101 32 0.9941798941798936 1101 33 2.479100529100535 1101 34 0.3169312169312164 1101 35 3.3470899470899482 1101 36 2.544973544973544 1101 37 3.7417989417989403 1101 38 3.6925925925926038 1101 39 3.058994708994703 1101 40 3.589682539682542 1101 41 1.2232804232804213 1101 42 1.7616402116402199 1101 43 0.8701058201058274 1101 44 3.322222222222223 1101 45 2.002380952380939 1101 46 4.427513227513231 1101 47 3.522486772486779 1101 48 3.813227513227517 1101 49 4.4261904761904844 1101 50 2.0357142857142847 1101 51 -4.18518518518518 2201 1 -9.414814814814804 2201 2 -5.202645502645495 2201 3 -0.17222222222222427 2201 4 1.2510582010581999 2201 5 -3.046560846560837 2201 6 0.175396825396831 2201 7 2.367724867724867 2201 8 2.365873015873021 2201 9 0.7010582010582027 2201 10 1.4507936507936563 2201 11 -4.150529100529106 2201 12 -0.24338624338624726 2201 13 1.1992063492063494 2201 14 3.2748677248677254 2201 15 2.6841269841269906 2201 16 3.242592592592594 2201 17 2.889153439153432 2201 18 -10.146560846560853 2201 19 -6.86507936507936 2201 20 -0.6629629629629505 2201 21 -2.160317460317451 2201 22 -7.0547619047618895 2201 23 -4.111904761904768 2201 24 -9.55185185185185 2201 25 -1.4637566137566083 2201 26 2.548677248677251 2201 27 3.3650793650793744 2201 28 -0.4835978835978807 2201 29 -2.8820105820105795 2201 30 -0.22566137566137456 2201 31 -9.707936507936509 2201 32 -0.9224867724867707 2201 33 2.8124338624338705 2201 34 1.8169312169312164 2201 35 -3.2362433862433875 2201 36 1.37830687830688 2201 37 -2.8415343915343954 2201 38 -3.390740740740732 2201 39 1.3923280423280389 2201 40 0.006349206349206327 2201 41 0.8899470899470856 2201 42 -3.321693121693116 2201 43 -4.546560846560837 2201 44 -3.0944444444444414 2201 45 0.41904761904760335 2201 46 -8.405820105820105 2201 47 -4.5608465608465565 2201 48 0.7298941798941811 2201 49 -4.157142857142851 2201 50 -6.047619047619051 2201 51 -2.268518518518512 2202 1 3.085185185185196 2202 2 -0.4526455026454954 2202 3 2.5777777777777757 2202 4 -0.24894179894180013 2202 5 3.203439153439163 2202 6 2.175396825396831 2202 7 2.367724867724867 2202 8 1.8658730158730208 2202 9 2.2010582010582027 2202 10 1.9507936507936563 2202 11 2.5994708994708944 2202 12 1.0066137566137527 2202 13 -3.5507936507936506 2202 14 -0.22513227513227463 2202 15 3.1841269841269906 2202 16 1.2425925925925938 2202 17 2.389153439153432 2202 18 -5.8965608465608526 2202 19 0.6349206349206398 2202 20 1.3370370370370495 2202 21 2.589682539682549 2202 22 1.4452380952381105 2202 23 -3.6119047619047677 2202 24 1.9481481481481495 2202 25 2.7862433862433917 2202 26 2.548677248677251 2202 27 3.8650793650793744 2202 28 3.2664021164021193 2202 29 3.8679894179894205 2202 30 3.5243386243386254 2202 31 0.542063492063491 2202 32 1.8275132275132293 2202 33 2.8124338624338705 2202 34 3.3169312169312164 2202 35 3.0137566137566125 2202 36 2.37830687830688 2202 37 3.9084656084656046 2202 38 -2.890740740740732 2202 39 -5.607671957671961 2202 40 0.006349206349206327 2202 41 2.8899470899470856 2202 42 -0.07169312169311581 2202 43 1.203439153439163 2202 44 0.9055555555555586 2202 45 2.1690476190476033 2202 46 2.594179894179895 2202 47 1.1891534391534435 2202 48 2.979894179894181 2202 49 3.5928571428571487 2202 50 2.702380952380949 2202 51 1.731481481481488 1102 1 0.08518518518519613 1102 2 2.5473544973545046 1102 3 -0.4222222222222243 1102 4 1.2510582010581999 1102 5 3.203439153439163 1102 6 2.675396825396831 1102 7 1.867724867724867 1102 8 1.3658730158730208 1102 9 2.2010582010582027 1102 10 -0.2992063492063437 1102 11 2.5994708994708944 1102 12 1.0066137566137527 1102 13 2.6992063492063494 1102 14 2.2748677248677254 1102 15 0.6841269841269906 1102 16 0.7425925925925938 1102 17 2.389153439153432 1102 18 2.3534391534391474 1102 19 2.6349206349206398 1102 20 1.3370370370370495 1102 21 -3.660317460317451 1102 22 0.44523809523811053 1102 23 2.6380952380952323 1102 24 3.4481481481481495 1102 25 0.7862433862433917 1102 26 2.548677248677251 1102 27 3.3650793650793744 1102 28 3.2664021164021193 1102 29 3.8679894179894205 1102 30 3.0243386243386254 1102 31 2.792063492063491 1102 32 1.8275132275132293 1102 33 2.8124338624338705 1102 34 3.8169312169312164 1102 35 2.5137566137566125 1102 36 2.87830687830688 1102 37 3.4084656084656046 1102 38 3.359259259259268 1102 39 3.892328042328039 1102 40 1.7563492063492063 1102 41 2.8899470899470856 1102 42 1.9283068783068842 1102 43 3.203439153439163 1102 44 2.1555555555555586 1102 45 2.1690476190476033 1102 46 2.594179894179895 1102 47 3.6891534391534435 1102 48 3.479894179894181 1102 49 2.0928571428571487 1102 50 3.702380952380949 1102 51 2.981481481481488 2203 1 3.085185185185196 2203 2 -0.852645502645494 2203 3 1.3777777777777729 2203 4 2.7510582010582 2203 5 -1.796560846560837 2203 6 -3.824603174603169 2203 7 0.9677248677248684 2203 8 1.665873015873018 2203 9 0.8010582010582041 2203 10 1.7507936507936535 2203 11 0.7994708994708972 2203 12 0.3066137566137499 2203 13 -0.5007936507936535 2203 14 1.4748677248677282 2203 15 -2.415873015873011 2203 16 -1.957407407407409 2203 17 3.089153439153435 2203 18 1.8534391534391474 2203 19 0.43492063492063693 2203 20 2.6370370370370466 2203 21 0.3896825396825463 2203 22 -1.0547619047618895 2203 23 1.2380952380952337 2203 24 1.6481481481481524 2203 25 2.9862433862433946 2203 26 2.348677248677248 2203 27 -1.8349206349206284 2203 28 2.5664021164021165 2203 29 3.1679894179894177 2203 30 1.924338624338624 2203 31 0.39206349206349245 2203 32 -1.4724867724867678 2203 33 0.21243386243386908 2203 34 -1.8830687830687864 2203 35 -6.786243386243388 2203 36 0.07830687830688277 2203 37 0.2084656084656018 2203 38 -4.240740740740733 2203 39 -2.2076719576719626 2203 40 2.2563492063492063 2203 41 2.6899470899470828 2203 42 2.0283068783068856 2203 43 1.8034391534391645 2203 44 3.055555555555557 2203 45 2.6690476190476033 2203 46 3.4941798941798936 2203 47 3.789153439153445 2203 48 2.479894179894181 2203 49 3.892857142857146 2203 50 1.9023809523809518 2203 51 -3.3185185185185126 1103 1 -1.9148148148148039 1103 2 1.3473544973545017 1103 3 2.5777777777777757 1103 4 2.7510582010582 1103 5 2.0034391534391602 1103 6 1.5753968253968296 1103 7 1.7677248677248656 1103 8 1.665873015873018 1103 9 2.4010582010582056 1103 10 0.9507936507936563 1103 11 0.7994708994708972 1103 12 2.30661375661375 1103 13 2.8992063492063522 1103 14 4.274867724867725 1103 15 1.784126984126992 1103 16 3.042592592592591 1103 17 2.6891534391534293 1103 18 3.0534391534391503 1103 19 2.8349206349206426 1103 20 3.0370370370370523 1103 21 2.789682539682552 1103 22 2.1452380952381134 1103 23 2.4380952380952294 1103 24 2.848148148148148 1103 25 2.9862433862433946 1103 26 3.1486772486772523 1103 27 3.1650793650793716 1103 28 2.5664021164021165 1103 29 3.5679894179894234 1103 30 2.3243386243386226 1103 31 2.992063492063494 1103 32 3.527513227513232 1103 33 3.412433862433872 1103 34 3.5169312169312192 1103 35 3.2137566137566154 1103 36 3.0783068783068828 1103 37 4.008465608465606 1103 38 3.9592592592592695 1103 39 3.9923280423280403 1103 40 3.456349206349209 1103 41 3.0899470899470884 1103 42 3.628306878306887 1103 43 2.6034391534391617 1103 44 2.6555555555555586 1103 45 3.869047619047606 1103 46 3.894179894179892 1103 47 3.3891534391534464 1103 48 4.079894179894183 1103 49 4.292857142857152 1103 50 3.902380952380952 1103 51 5.0814814814814895 2204 1 1.5851851851851961 2204 2 -5.202645502645495 2204 3 1.0777777777777757 2204 4 -3.4989417989418 2204 5 -6.046560846560837 2204 6 0.175396825396831 2204 7 -2.632275132275133 2204 8 1.3658730158730208 2204 9 -1.0489417989417973 2204 10 -1.7992063492063437 2204 11 0.5994708994708944 2204 12 0.5066137566137527 2204 13 0.6992063492063494 2204 14 1.7748677248677254 2204 15 3.1841269841269906 2204 16 -4.507407407407406 2204 17 2.889153439153432 2204 18 2.8534391534391474 2204 19 3.1349206349206398 2204 20 3.3370370370370495 2204 21 3.089682539682549 2204 22 1.4452380952381105 2204 23 3.1380952380952323 2204 24 -2.8018518518518505 2204 25 2.2862433862433917 2204 26 1.0486772486772509 2204 27 3.3650793650793744 2204 28 2.7664021164021193 2204 29 3.3679894179894205 2204 30 2.5243386243386254 2204 31 2.792063492063491 2204 32 1.3275132275132293 2204 33 2.3124338624338705 2204 34 3.3169312169312164 2204 35 3.0137566137566125 2204 36 2.87830687830688 2204 37 2.9084656084656046 2204 38 3.859259259259268 2204 39 3.892328042328039 2204 40 3.2563492063492063 2204 41 1.3899470899470856 2204 42 3.928306878306884 2204 43 3.203439153439163 2204 44 1.1555555555555586 2204 45 1.6690476190476033 2204 46 2.594179894179895 2204 47 1.1891534391534435 2204 48 2.979894179894181 2204 49 3.5928571428571487 2204 50 2.202380952380949 2204 51 2.481481481481488 2205 1 1.0851851851851961 2205 2 2.5473544973545046 2205 3 2.5777777777777757 2205 4 0.7510582010581999 2205 5 1.203439153439163 2205 6 1.8420634920634953 2205 7 2.7010582010582027 2205 8 2.1992063492063565 2205 9 0.5343915343915384 2205 10 1.6174603174603206 2205 11 2.2661375661375587 2205 12 1.5066137566137527 2205 13 2.3658730158730137 2205 14 -5.3917989417989425 2205 15 -5.482539682539677 2205 16 -0.0907407407407419 2205 17 1.2224867724867678 2205 18 2.5201058201058117 2205 19 -0.36507936507936023 2205 20 2.5037037037037138 2205 21 0.9230158730158848 2205 22 1.6119047619047748 2205 23 0.3047619047618966 2205 24 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3.965079365079376 1208 28 3.3664021164021207 1208 29 3.5679894179894234 1208 30 3.5243386243386254 1208 31 3.3920634920634924 1208 32 4.327513227513229 1208 33 3.412433862433872 1208 34 3.5169312169312192 1208 35 2.8137566137566097 1208 36 2.2783068783068785 1208 37 3.208465608465602 1208 38 3.159259259259265 1208 39 3.5923280423280417 1208 40 3.456349206349209 1208 41 3.489947089947087 1208 42 4.428306878306884 1208 43 3.403439153439166 1208 44 2.25555555555556 1208 45 3.069047619047602 1208 46 3.4941798941798936 1208 47 2.9891534391534407 1208 48 3.679894179894184 1208 49 2.69285714285715 1208 50 3.902380952380952 1208 51 2.481481481481488 1209 1 1.5851851851851961 1209 2 1.0473544973545046 1209 3 1.0777777777777757 1209 4 2.7510582010582 1209 5 1.703439153439163 1209 6 0.675396825396831 1209 7 2.367724867724867 1209 8 1.8658730158730208 1209 9 2.2010582010582027 1209 10 0.9507936507936563 1209 11 2.5994708994708944 1209 12 2.5066137566137527 1209 13 2.1992063492063494 1209 14 2.2748677248677254 1209 15 3.1841269841269906 1209 16 3.742592592592594 1209 17 3.389153439153432 1209 18 3.3534391534391474 1209 19 3.6349206349206398 1209 20 2.8370370370370495 1209 21 2.589682539682549 1209 22 1.9452380952381105 1209 23 2.6380952380952323 1209 24 4.4481481481481495 1209 25 2.7862433862433917 1209 26 2.048677248677251 1209 27 2.3650793650793744 1209 28 2.2664021164021193 1209 29 3.3679894179894205 1209 30 3.0243386243386254 1209 31 2.292063492063491 1209 32 3.3275132275132293 1209 33 1.3124338624338705 1209 34 3.3169312169312164 1209 35 3.0137566137566125 1209 36 -3.87169312169312 1209 37 1.1584656084656046 1209 38 3.859259259259268 1209 39 3.892328042328039 1209 40 2.7563492063492063 1209 41 1.8899470899470856 1209 42 2.928306878306884 1209 43 3.203439153439163 1209 44 3.6555555555555586 1209 45 3.6690476190476033 1209 46 3.594179894179895 1209 47 3.1891534391534435 1209 48 3.479894179894181 1209 49 -2.6571428571428513 1209 50 3.202380952380949 1209 51 0.7314814814814881 1214 1 -1.1648148148148039 1214 2 -1.7026455026454954 1214 3 -3.6722222222222243 1214 4 -3.4989417989418 1214 5 1.703439153439163 1214 6 1.175396825396831 1214 7 1.867724867724867 1214 8 -4.384126984126979 1214 9 -4.048941798941797 1214 10 -0.7992063492063437 1214 11 0.3494708994708944 1214 12 -3.2433862433862473 1214 13 0.4492063492063494 1214 14 -3.4751322751322746 1214 15 1.1841269841269906 1214 16 1.7425925925925938 1214 17 -0.36084656084656785 1214 18 3.3534391534391474 1214 19 -3.6150793650793602 1214 20 -3.4129629629629505 1214 21 -3.660317460317451 1214 22 -1.3047619047618895 1214 23 0.3880952380952323 1214 24 0.6981481481481495 1214 25 -3.4637566137566083 1214 26 1.0486772486772509 1214 27 3.3650793650793744 1214 28 -3.9835978835978807 1214 29 1.1179894179894205 1214 30 -3.2256613756613746 1214 31 -2.957936507936509 1214 32 1.0775132275132293 1214 33 -2.9375661375661295 1214 34 -2.4330687830687836 1214 35 -2.7362433862433875 1214 36 0.12830687830687992 1214 37 0.15846560846560465 1214 38 3.359259259259268 1214 39 1.8923280423280389 1214 40 3.7563492063492063 1214 41 1.8899470899470856 1214 42 3.928306878306884 1214 43 3.203439153439163 1214 44 3.1555555555555586 1214 45 3.6690476190476033 1214 46 4.594179894179895 1214 47 3.6891534391534435 1214 48 3.979894179894181 1214 49 -1.6571428571428513 1214 50 -2.047619047619051 1214 51 -2.268518518518512 1215 1 0.08518518518519613 1215 2 2.5473544973545046 1215 3 -0.4222222222222243 1215 4 2.7510582010582 1215 5 3.203439153439163 1215 6 3.175396825396831 1215 7 2.367724867724867 1215 8 2.865873015873021 1215 9 3.2010582010582027 1215 10 1.9507936507936563 1215 11 2.5994708994708944 1215 12 3.5066137566137527 1215 13 3.6992063492063494 1215 14 4.274867724867725 1215 15 3.1841269841269906 1215 16 4.242592592592594 1215 17 2.889153439153432 1215 18 3.8534391534391474 1215 19 2.6349206349206398 1215 20 2.8370370370370495 1215 21 2.589682539682549 1215 22 -1.0547619047618895 1215 23 2.6380952380952323 1215 24 -0.5518518518518505 1215 25 2.7862433862433917 1215 26 2.548677248677251 1215 27 3.3650793650793744 1215 28 3.7664021164021193 1215 29 4.3679894179894205 1215 30 1.5243386243386254 1215 31 3.792063492063491 1215 32 4.327513227513229 1215 33 2.8124338624338705 1215 34 3.3169312169312164 1215 35 4.0137566137566125 1215 36 2.87830687830688 1215 37 3.4084656084656046 1215 38 3.359259259259268 1215 39 3.392328042328039 1215 40 -0.7436507936507937 1215 41 2.8899470899470856 1215 42 3.428306878306884 1215 43 3.203439153439163 1215 44 4.6555555555555586 1215 45 3.6690476190476033 1215 46 4.094179894179895 1215 47 3.1891534391534435 1215 48 3.479894179894181 1215 49 4.092857142857149 1215 50 3.702380952380949 1215 51 4.481481481481488 1212 1 -0.9148148148148039 1212 2 0.5473544973545046 1212 3 0.5777777777777757 1212 4 -1.2489417989418001 1212 5 3.203439153439163 1212 6 -7.157936507936501 1212 7 -0.632275132275133 1212 8 -3.134126984126979 1212 9 -0.7989417989417973 1212 10 -7.382539682539676 1212 11 2.93280423280423 1212 12 1.5066137566137527 1212 13 0.3658730158730137 1212 14 -1.0584656084656103 1212 15 0.8507936507936549 1212 16 2.909259259259258 1212 17 0.5558201058200964 1212 18 3.186772486772483 1212 19 0.30158730158730407 1212 20 0.5037037037037138 1212 21 2.2563492063492134 1212 22 1.6119047619047748 1212 23 1.6380952380952323 1212 24 2.4481481481481495 1212 25 1.7862433862433917 1212 26 2.215343915343915 1212 27 3.0317460317460387 1212 28 1.7664021164021193 1212 29 0.36798941798942053 1212 30 -8.142328042328042 1212 31 -1.8746031746031733 1212 32 2.9941798941798936 1212 33 0.4791005291005348 1212 34 -6.016402116402116 1212 35 2.0137566137566125 1212 36 1.87830687830688 1212 37 -3.5915343915343954 1212 38 2.359259259259268 1212 39 0.39232804232803886 1212 40 -7.4103174603174615 1212 41 2.55661375661375 1212 42 3.0949735449735485 1212 43 2.203439153439163 1212 44 3.322222222222223 1212 45 -4.9976190476190645 1212 46 0.09417989417989503 1212 47 0.18915343915344351 1212 48 0.47989417989418115 1212 49 1.0928571428571487 1212 50 -0.9642857142857153 1212 51 3.481481481481488 1213 1 3.085185185185196 1213 2 2.5473544973545046 1213 3 2.5777777777777757 1213 4 2.7510582010582 1213 5 -2.796560846560837 1213 6 1.175396825396831 1213 7 1.367724867724867 1213 8 0.8658730158730208 1213 9 3.2010582010582027 1213 10 0.9507936507936563 1213 11 1.5994708994708944 1213 12 3.5066137566137527 1213 13 1.6992063492063494 1213 14 4.274867724867725 1213 15 4.184126984126991 1213 16 4.242592592592594 1213 17 3.889153439153432 1213 18 3.8534391534391474 1213 19 3.6349206349206398 1213 20 3.8370370370370495 1213 21 1.5896825396825491 1213 22 0.9452380952381105 1213 23 1.6380952380952323 1213 24 2.4481481481481495 1213 25 1.7862433862433917 1213 26 1.5486772486772509 1213 27 2.3650793650793744 1213 28 1.7664021164021193 1213 29 -3.6320105820105795 1213 30 1.5243386243386254 1213 31 1.792063492063491 1213 32 -3.6724867724867707 1213 33 1.8124338624338705 1213 34 2.3169312169312164 1213 35 4.0137566137566125 1213 36 1.87830687830688 1213 37 2.4084656084656046 1213 38 2.359259259259268 1213 39 2.392328042328039 1213 40 -3.7436507936507937 1213 41 1.8899470899470856 1213 42 2.428306878306884 1213 43 4.203439153439163 1213 44 4.6555555555555586 1213 45 2.6690476190476033 1213 46 3.094179894179895 1213 47 2.1891534391534435 1213 48 4.479894179894181 1213 49 5.092857142857149 1213 50 4.702380952380949 1213 51 5.481481481481488 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 5.0 23 5.0 24 6.0 25 21.0 26 28.0 27 34.0 28 39.0 29 58.0 30 83.0 31 90.0 32 95.0 33 119.0 34 130.0 35 157.0 36 212.0 37 247.0 38 417.0 39 542.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.993006993006993 17.613636363636363 13.286713286713287 37.10664335664335 2 11.888111888111888 22.377622377622377 44.84265734265735 20.89160839160839 3 17.43881118881119 24.388111888111887 37.019230769230774 21.153846153846153 4 11.276223776223777 27.36013986013986 35.48951048951049 25.874125874125873 5 11.844405594405595 35.66433566433567 41.47727272727273 11.013986013986015 6 26.442307692307693 44.53671328671329 17.613636363636363 11.407342657342658 7 22.15909090909091 32.47377622377623 20.236013986013987 25.13111888111888 8 21.022727272727273 42.43881118881119 20.97902097902098 15.55944055944056 9 22.02797202797203 16.826923076923077 20.585664335664337 40.55944055944056 10 15.209790209790212 35.31468531468531 29.23951048951049 20.236013986013987 11 29.50174825174825 21.678321678321677 32.38636363636363 16.433566433566433 12 19.318181818181817 24.78146853146853 29.938811188811187 25.961538461538463 13 24.3006993006993 19.58041958041958 35.22727272727273 20.89160839160839 14 17.43881118881119 21.284965034965033 27.753496503496507 33.52272727272727 15 20.8479020979021 27.185314685314687 22.85839160839161 29.10839160839161 16 29.50174825174825 26.87937062937063 24.344405594405593 19.274475524475523 17 20.017482517482517 36.01398601398601 25.34965034965035 18.61888111888112 18 27.972027972027973 26.223776223776223 27.666083916083917 18.138111888111887 19 21.94055944055944 25.34965034965035 25.437062937062937 27.27272727272727 20 20.54195804195804 24.16958041958042 25.874125874125873 29.414335664335667 21 19.230769230769234 27.316433566433567 25.961538461538463 27.49125874125874 22 20.367132867132867 33.82867132867133 24.65034965034965 21.153846153846153 23 20.27972027972028 26.617132867132863 25.174825174825177 27.928321678321677 24 28.671328671328673 27.22902097902098 25.0 19.09965034965035 25 19.842657342657343 34.35314685314685 27.185314685314687 18.61888111888112 26 28.146853146853147 27.753496503496507 25.524475524475527 18.575174825174827 27 19.973776223776223 34.96503496503497 26.354895104895103 18.706293706293707 28 18.75 24.825174825174827 27.972027972027973 28.452797202797203 29 21.765734265734267 27.797202797202797 30.375874125874127 20.06118881118881 30 22.333916083916083 30.506993006993007 27.14160839160839 20.017482517482517 31 21.197552447552447 29.545454545454547 27.578671328671327 21.678321678321677 32 19.79895104895105 29.326923076923077 29.37062937062937 21.503496503496503 33 19.93006993006993 29.1520979020979 30.419580419580424 20.498251748251747 34 20.1486013986014 28.015734265734267 31.687062937062937 20.1486013986014 35 19.842657342657343 29.72027972027972 28.583916083916083 21.853146853146853 36 19.93006993006993 28.146853146853147 30.463286713286713 21.45979020979021 37 18.706293706293707 32.29895104895105 28.190559440559444 20.804195804195803 38 19.055944055944053 23.426573426573427 37.10664335664335 20.41083916083916 39 18.356643356643357 21.765734265734267 29.676573426573427 30.201048951048953 40 17.045454545454543 20.323426573426573 38.8986013986014 23.732517482517483 41 18.312937062937063 22.596153846153847 28.62762237762238 30.463286713286713 42 25.61188811188811 23.164335664335663 31.293706293706293 19.93006993006993 43 17.744755244755243 22.63986013986014 37.45629370629371 22.15909090909091 44 15.646853146853147 32.12412587412587 31.16258741258741 21.066433566433567 45 15.384615384615385 25.393356643356647 37.15034965034965 22.071678321678323 46 24.125874125874127 23.20804195804196 29.50174825174825 23.164335664335663 47 16.04020979020979 22.508741258741257 28.452797202797203 32.99825174825175 48 15.646853146853147 23.33916083916084 28.190559440559444 32.82342657342657 49 24.125874125874127 22.77097902097902 29.28321678321678 23.81993006993007 50 15.166083916083917 23.33916083916084 38.06818181818182 23.426573426573427 51 13.986013986013987 21.94055944055944 30.244755244755243 33.82867132867133 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 1.0 12 2.0 13 2.0 14 2.0 15 2.0 16 2.0 17 4.0 18 6.0 19 6.0 20 6.0 21 5.0 22 4.0 23 3.5 24 3.0 25 11.0 26 23.0 27 27.0 28 36.5 29 46.0 30 64.5 31 83.0 32 95.0 33 107.0 34 129.5 35 152.0 36 176.5 37 201.0 38 209.0 39 217.0 40 205.0 41 193.0 42 176.5 43 160.0 44 150.0 45 140.0 46 161.5 47 183.0 48 180.0 49 177.0 50 168.5 51 160.0 52 136.5 53 113.0 54 90.0 55 67.0 56 67.5 57 68.0 58 52.0 59 36.0 60 37.0 61 38.0 62 31.0 63 24.0 64 20.5 65 17.0 66 12.5 67 8.0 68 8.5 69 9.0 70 8.0 71 7.0 72 6.5 73 6.0 74 4.0 75 2.0 76 2.0 77 1.5 78 1.0 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2288.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.13286713286713 #Duplication Level Percentage of deduplicated Percentage of total 1 98.38709677419355 90.64685314685315 2 0.6166982922201139 1.1363636363636365 3 0.09487666034155598 0.26223776223776224 4 0.2846299810246679 1.048951048951049 5 0.04743833017077799 0.21853146853146854 6 0.04743833017077799 0.26223776223776224 7 0.04743833017077799 0.30594405594405594 8 0.0 0.0 9 0.09487666034155598 0.7867132867132868 >10 0.3795066413662239 5.332167832167832 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC 26 1.1363636363636365 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC 20 0.8741258741258742 No Hit CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC 19 0.8304195804195804 RNA PCR Primer, Index 39 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC 13 0.5681818181818182 RNA PCR Primer, Index 45 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC 12 0.5244755244755245 No Hit CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC 11 0.4807692307692308 No Hit CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC 11 0.4807692307692308 No Hit CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC 10 0.4370629370629371 RNA PCR Primer, Index 23 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC 9 0.3933566433566434 No Hit CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC 9 0.3933566433566434 No Hit CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC 7 0.30594405594405594 No Hit CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC 6 0.26223776223776224 No Hit CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC 5 0.21853146853146854 No Hit CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC 4 0.17482517482517482 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC 4 0.17482517482517482 No Hit CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC 4 0.17482517482517482 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCG 4 0.17482517482517482 No Hit TCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGT 4 0.17482517482517482 No Hit TCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT 4 0.17482517482517482 No Hit CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC 3 0.13111888111888112 No Hit CTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTC 3 0.13111888111888112 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.043706293706293704 7 0.0 0.0 0.043706293706293704 8 0.0 0.0 0.08741258741258741 9 0.0 0.0 0.08741258741258741 10 0.0 0.0 0.08741258741258741 11 0.0 0.0 0.08741258741258741 12 0.0 0.0 0.08741258741258741 13 0.0 0.0 0.08741258741258741 14 0.0 0.0 0.08741258741258741 15 0.0 0.0 0.08741258741258741 16 0.0 0.0 0.08741258741258741 17 0.0 0.0 0.08741258741258741 18 0.0 0.0 0.08741258741258741 19 0.0 0.0 0.08741258741258741 20 0.0 0.0 0.08741258741258741 21 0.0 0.0 0.08741258741258741 22 0.0 0.0 0.13111888111888112 23 0.0 0.0 0.17482517482517482 24 0.0 0.0 0.17482517482517482 25 0.0 0.0 0.17482517482517482 26 0.0 0.0 0.21853146853146854 27 0.0 0.0 0.26223776223776224 28 0.0 0.0 0.34965034965034963 29 0.0 0.0 0.4807692307692308 30 0.0 0.0 0.8741258741258742 31 0.0 0.0 1.7919580419580419 32 0.0 0.0 2.4912587412587412 33 0.0 0.0 3.5402097902097904 34 0.0 0.0 5.288461538461538 35 0.0 0.0 6.993006993006993 36 0.0 0.0 8.828671328671328 37 0.0 0.0 10.62062937062937 38 0.0 0.0 12.5 39 0.0 0.0 14.729020979020978 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCTCGT 30 1.3506906E-6 44.999996 37 CATCTCC 30 1.3506906E-6 44.999996 9 TCTCGTA 30 1.3506906E-6 44.999996 38 TCGTATG 30 1.3506906E-6 44.999996 40 CGAGACT 25 2.7843895E-5 44.999996 23 CTCGTAT 30 1.3506906E-6 44.999996 39 AGCCCAC 35 3.9088063E-6 38.571426 17 GTATGCC 35 3.9088063E-6 38.571426 42 ATCTCCG 35 3.9088063E-6 38.571426 10 CACATCT 35 3.9088063E-6 38.571426 7 GCCCACG 35 3.9088063E-6 38.571426 18 TGCCGTC 35 3.9088063E-6 38.571426 45 CCACGAG 35 3.9088063E-6 38.571426 20 TATGCCG 35 3.9088063E-6 38.571426 43 GAGCCCA 35 3.9088063E-6 38.571426 16 CACGAGA 35 3.9088063E-6 38.571426 21 CCCACGA 35 3.9088063E-6 38.571426 19 ACATCTC 35 3.9088063E-6 38.571426 8 ACACATC 35 3.9088063E-6 38.571426 6 ACGAGAC 35 3.9088063E-6 38.571426 22 >>END_MODULE