H23NVBCXX l02n01 18h_95.341000000054c7.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23NVBCXX l02n01 18h_95.341000000054c7.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2288
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC	26	1.1363636363636365	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC	20	0.8741258741258742	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC	19	0.8304195804195804	RNA PCR Primer, Index 39 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC	13	0.5681818181818182	RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC	12	0.5244755244755245	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC	11	0.4807692307692308	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC	11	0.4807692307692308	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC	10	0.4370629370629371	RNA PCR Primer, Index 23 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC	9	0.3933566433566434	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC	9	0.3933566433566434	No Hit
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC	7	0.30594405594405594	No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC	6	0.26223776223776224	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC	5	0.21853146853146854	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC	4	0.17482517482517482	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC	4	0.17482517482517482	No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC	4	0.17482517482517482	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCG	4	0.17482517482517482	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGT	4	0.17482517482517482	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT	4	0.17482517482517482	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC	3	0.13111888111888112	No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTC	3	0.13111888111888112	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGT	30	1.3506906E-6	44.999996	37
CATCTCC	30	1.3506906E-6	44.999996	9
TCTCGTA	30	1.3506906E-6	44.999996	38
TCGTATG	30	1.3506906E-6	44.999996	40
CGAGACT	25	2.7843895E-5	44.999996	23
CTCGTAT	30	1.3506906E-6	44.999996	39
AGCCCAC	35	3.9088063E-6	38.571426	17
GTATGCC	35	3.9088063E-6	38.571426	42
ATCTCCG	35	3.9088063E-6	38.571426	10
CACATCT	35	3.9088063E-6	38.571426	7
GCCCACG	35	3.9088063E-6	38.571426	18
TGCCGTC	35	3.9088063E-6	38.571426	45
CCACGAG	35	3.9088063E-6	38.571426	20
TATGCCG	35	3.9088063E-6	38.571426	43
GAGCCCA	35	3.9088063E-6	38.571426	16
CACGAGA	35	3.9088063E-6	38.571426	21
CCCACGA	35	3.9088063E-6	38.571426	19
ACATCTC	35	3.9088063E-6	38.571426	8
ACACATC	35	3.9088063E-6	38.571426	6
ACGAGAC	35	3.9088063E-6	38.571426	22

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content