FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_94.341000000054ba.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_94.341000000054ba.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115451
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTC4900.42442248226520346No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1620.14031926964686317No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAATC750.041.9987933
ATCTCGT850.039.7047437
CTCGTAT850.039.7047439
TCTCGTA850.039.7047438
GCAATCT750.038.99887534
CCCACGA950.037.89364219
TATGCCG900.037.49891743
GACCAGC850.037.05775526
GTGTAAC250.002090663636.0301671
AGCCCAC1000.035.9989617
CCGAGCC1000.035.9989614
ACGAGAC1000.035.9989622
CCACGAG1000.035.9989620
GTATGCC950.035.5252942
CGAGACC900.034.9989923
GCCCACG1050.034.28472518
CACGAGA1050.034.28472521
CGAGCCC1050.034.28472515
TGCCGTC1000.033.7636545
ATGCCGT1000.033.74902344
Produced by FastQC (version 0.11.2)