##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 18h_93.341000000054ae.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3387 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.550634780041335 38.0 32.0 38.0 27.0 38.0 2 34.92353114850901 38.0 32.0 38.0 32.0 38.0 3 34.96870386772955 38.0 32.0 38.0 32.0 38.0 4 35.18836728668438 38.0 32.0 38.0 32.0 38.0 5 35.399468556244464 38.0 32.0 38.0 32.0 38.0 6 36.9728373191615 38.0 38.0 40.0 32.0 40.0 7 36.57395925597874 38.0 38.0 40.0 32.0 40.0 8 36.462060820785354 38.0 38.0 40.0 32.0 40.0 9 36.468556244464125 38.0 38.0 40.0 32.0 40.0 10 36.328904635370534 38.0 38.0 40.0 32.0 40.0 11 36.664009447888986 38.0 38.0 40.0 32.0 40.0 12 36.15323294951284 38.0 38.0 40.0 32.0 40.0 13 36.51372896368468 38.0 38.0 40.0 32.0 40.0 14 36.118098612341306 38.0 38.0 40.0 32.0 40.0 15 36.349571892530264 38.0 38.0 40.0 32.0 40.0 16 36.1175081192796 38.0 38.0 40.0 32.0 40.0 17 35.927664599940954 38.0 38.0 40.0 27.0 40.0 18 35.96014171833481 38.0 38.0 40.0 27.0 40.0 19 36.126365515205194 38.0 38.0 40.0 32.0 40.0 20 36.00059049306171 38.0 38.0 40.0 27.0 40.0 21 36.214939474461175 38.0 38.0 40.0 32.0 40.0 22 36.289046353705345 38.0 38.0 40.0 32.0 40.0 23 36.14319456746383 38.0 38.0 40.0 32.0 40.0 24 36.14821375848834 38.0 38.0 40.0 27.0 40.0 25 36.03838204901093 38.0 38.0 40.0 27.0 40.0 26 35.715382344257456 38.0 38.0 40.0 27.0 40.0 27 35.84351933864777 38.0 38.0 40.0 27.0 40.0 28 35.88780631827576 38.0 38.0 40.0 27.0 40.0 29 35.92175966932388 38.0 38.0 40.0 27.0 40.0 30 36.051077649837616 38.0 38.0 40.0 27.0 40.0 31 35.812223206377325 38.0 38.0 40.0 27.0 40.0 32 35.98287570121051 38.0 38.0 40.0 27.0 40.0 33 35.66017124298789 38.0 38.0 40.0 27.0 40.0 34 35.877177443165046 38.0 38.0 40.0 27.0 40.0 35 35.87215825214054 38.0 38.0 40.0 27.0 40.0 36 35.82757602598169 38.0 38.0 40.0 27.0 40.0 37 35.81399468556244 38.0 38.0 40.0 27.0 40.0 38 35.889873043991734 38.0 38.0 40.0 27.0 40.0 39 35.7012105107765 38.0 38.0 40.0 27.0 40.0 40 35.655447298494245 38.0 38.0 40.0 27.0 40.0 41 35.61706524948332 38.0 38.0 40.0 27.0 40.0 42 35.857691172128725 38.0 38.0 40.0 27.0 40.0 43 35.7599645704163 38.0 38.0 40.0 27.0 40.0 44 35.58193091231178 38.0 38.0 40.0 27.0 40.0 45 35.743726011219366 38.0 38.0 40.0 27.0 40.0 46 35.5388249188072 38.0 38.0 40.0 27.0 40.0 47 35.49306170652495 38.0 38.0 40.0 27.0 40.0 48 35.61883672866844 38.0 38.0 40.0 27.0 40.0 49 35.602598169471506 38.0 38.0 40.0 27.0 40.0 50 35.66135222911131 38.0 38.0 40.0 27.0 40.0 51 34.822261588426336 38.0 32.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2201 1 2.1104724702380935 2201 2 1.2698288690476218 2201 3 1.5147879464285765 2201 4 1.6747209821428655 2201 5 1.0117931547619108 2201 6 1.739508928571425 2201 7 2.848660714285721 2201 8 0.21850818452380594 2201 9 2.112574404761908 2201 10 2.1809709821428456 2201 11 2.390104166666667 2201 12 1.4385230654761827 2201 13 2.1642485119047663 2201 14 3.534858630952378 2201 15 3.2640625000000014 2201 16 3.3011904761904773 2201 17 3.167243303571418 2201 18 2.8707217261904745 2201 19 3.4249813988095212 2201 20 3.174293154761898 2201 21 2.0108630952380864 2201 22 2.3331845238095212 2201 23 2.1376116071428584 2201 24 3.1070498511904745 2201 25 1.2796130952381048 2201 26 1.4655133928571402 2201 27 3.9375372023809447 2201 28 4.017578125 2201 29 4.343824404761911 2201 30 3.6707961309523824 2201 31 3.057589285714286 2201 32 2.322451636904752 2201 33 2.2579055059523867 2201 34 3.648214285714282 2201 35 -0.18444940476190652 2201 36 2.464769345238089 2201 37 2.959765625000003 2201 38 2.912834821428561 2201 39 3.280766369047626 2201 40 3.2652901785714334 2201 41 3.119828869047609 2201 42 2.1216889880952436 2201 43 2.213616071428568 2201 44 0.858165922619051 2201 45 2.7985305059523924 2201 46 3.503924851190476 2201 47 4.047488839285705 2201 48 0.6234933035714221 2201 49 2.5680059523809504 2201 50 3.417503720238095 2201 51 3.7303199404761926 1101 1 0.010472470238092058 1101 2 0.36982886904762324 1101 3 -0.5852120535714249 1101 4 -4.225279017857133 1101 5 -2.0882068452380906 1101 6 1.6395089285714235 1101 7 2.2486607142857196 1101 8 1.3185081845238074 1101 9 0.8125744047619108 1101 10 -0.8190290178571544 1101 11 -0.6098958333333329 1101 12 -1.4614769345238159 1101 13 -0.03575148809523654 1101 14 -2.965141369047622 1101 15 -0.6359374999999972 1101 16 -2.3988095238095255 1101 17 2.0672433035714164 1101 18 1.470721726190476 1101 19 2.32498139880952 1101 20 1.3742931547619008 1101 21 -2.6891369047619165 1101 22 1.033184523809524 1101 23 1.6376116071428584 1101 24 -3.292950148809524 1101 25 0.27961309523810485 1101 26 1.665513392857143 1101 27 -0.5624627976190553 1101 28 -3.282421874999997 1101 29 1.0438244047619136 1101 30 2.570796130952381 1101 31 0.05758928571428612 1101 32 1.522451636904755 1101 33 3.1579055059523853 1101 34 3.0482142857142804 1101 35 -2.284449404761908 1101 36 -0.9352306547619094 1101 37 2.0597656250000043 1101 38 -2.9871651785714377 1101 39 0.9807663690476289 1101 40 2.665290178571432 1101 41 1.8198288690476119 1101 42 1.721688988095245 1101 43 -1.9863839285714349 1101 44 0.358165922619051 1101 45 -0.2014694940476076 1101 46 1.7039248511904788 1101 47 3.447488839285704 1101 48 3.5234933035714207 1101 49 1.7680059523809533 1101 50 0.8175037202380935 1101 51 -0.9696800595238102 2202 1 0.18190104166666288 2202 2 0.19840029761905242 2202 3 -0.2709263392857082 2202 4 -2.968136160714277 2202 5 0.7975074404761955 2202 6 1.8109374999999943 2202 7 2.705803571428575 2202 8 2.0042224702380906 2202 9 -1.9588541666666615 2202 10 -1.5333147321428697 2202 11 1.1043898809523824 2202 12 0.5099516369047521 2202 13 -2.692894345238088 2202 14 -3.1079985119047677 2202 15 -0.23593749999999858 2202 16 -0.19880952380952266 2202 17 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3 0.6147879464285779 1102 4 1.9747209821428626 1102 5 0.1117931547619122 1102 6 1.6395089285714235 1102 7 3.048660714285724 1102 8 0.9185081845238088 1102 9 0.4125744047619051 1102 10 1.5809709821428442 1102 11 -0.40989583333333 1102 12 -2.6614769345238187 1102 13 -0.6357514880952309 1102 14 -1.3651413690476204 1102 15 1.3640625000000028 1102 16 1.0011904761904802 1102 17 0.8672433035714207 1102 18 2.6707217261904717 1102 19 1.5249813988095227 1102 20 -2.825706845238102 1102 21 2.3108630952380835 1102 22 1.033184523809524 1102 23 1.6376116071428584 1102 24 1.707049851190476 1102 25 1.4796130952381077 1102 26 2.865513392857139 1102 27 3.6375372023809476 1102 28 2.517578125 1102 29 2.0438244047619136 1102 30 2.570796130952381 1102 31 3.257589285714289 1102 32 3.522451636904755 1102 33 3.557905505952384 1102 34 2.648214285714282 1102 35 1.915550595238095 1102 36 3.2647693452380864 1102 37 0.8597656250000014 1102 38 2.8128348214285595 1102 39 1.980766369047629 1102 40 0.665290178571432 1102 41 2.619828869047609 1102 42 0.12168898809524364 1102 43 1.4136160714285708 1102 44 1.9581659226190524 1102 45 0.5985305059523895 1102 46 -2.8960751488095227 1102 47 1.6474888392857068 1102 48 0.5234933035714207 1102 49 -0.8319940476190482 1102 50 -4.782496279761904 1102 51 2.4303199404761955 2203 1 -1.5323846726190524 2203 2 -4.087313988095236 2203 3 0.5862165178571459 2203 4 -8.253850446428562 2203 5 -1.6310639880952351 2203 6 -0.4747767857142904 2203 7 -7.865624999999994 2203 8 -2.7100632440476247 2203 9 -0.9588541666666615 2203 10 0.3238281249999915 2203 11 -7.4670386904761905 2203 12 -1.347191220238102 2203 13 0.5928199404761969 2203 14 -2.1079985119047677 2203 15 -5.950223214285714 2203 16 0.37261904761904674 2203 17 -2.475613839285728 2203 18 -1.1292782738095255 2203 19 -1.0750186011904788 2203 20 3.031436011904752 2203 21 -1.7748511904761983 2203 22 -2.5953869047619094 2203 23 0.20904017857142776 2203 24 1.8213355654761898 2203 25 -1.863244047619041 2203 26 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1.192633928571432 1208 16 0.37261904761904674 1208 17 0.6672433035714178 1208 18 1.1564360119047592 1208 19 -3.2178757440476247 1208 20 -4.111421130952387 1208 21 2.6537202380952323 1208 22 -1.4525297619047635 1208 23 -0.7909598214285722 1208 24 1.678478422619044 1208 25 -1.5775297619047493 1208 26 0.8940848214285708 1208 27 -2.4196056547619094 1208 28 -3.7681361607142847 1208 29 -0.4418898809523739 1208 30 0.599367559523813 1208 31 -3.3709821428571445 1208 32 -3.1061197916666785 1208 33 -1.9563802083333286 1208 34 -1.2089285714285722 1208 35 -1.5415922619047606 1208 36 -2.392373511904765 1208 37 -2.1116629464285666 1208 38 -0.5871651785714391 1208 39 -0.7906622023809433 1208 40 2.551004464285718 1208 41 1.9055431547618937 1208 42 2.1216889880952436 1208 43 2.213616071428568 1208 44 -3.9275483630952337 1208 45 -3.3443266369047535 1208 46 0.6467819940476218 1208 47 -1.8096540178571487 1208 48 0.8377790178571374 1208 49 -0.14627976190476488 1208 50 -0.5824962797619051 1208 51 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2.959765625000003 1209 38 -0.08716517857143913 1209 39 -0.9692336309523739 1209 40 2.7652901785714334 1209 41 3.119828869047609 1209 42 1.1216889880952436 1209 43 1.713616071428568 1209 44 1.358165922619051 1209 45 -0.2014694940476076 1209 46 1.503924851190476 1209 47 0.04748883928570535 1209 48 1.123493303571422 1209 49 3.0680059523809504 1209 50 -0.0824962797619051 1209 51 -2.5196800595238074 1214 1 3.6104724702380935 1214 2 -4.980171130952378 1214 3 0.014787946428576504 1214 4 1.6747209821428655 1214 5 -3.738206845238089 1214 6 -0.010491071428575083 1214 7 1.848660714285721 1214 8 1.218508184523806 1214 9 2.612574404761908 1214 10 1.6809709821428456 1214 11 2.390104166666667 1214 12 1.4385230654761827 1214 13 1.6642485119047663 1214 14 1.5348586309523782 1214 15 1.2640625000000014 1214 16 1.3011904761904773 1214 17 1.6672433035714178 1214 18 1.3707217261904745 1214 19 2.4249813988095212 1214 20 3.174293154761898 1214 21 1.0108630952380864 1214 22 1.3331845238095212 1214 23 2.1376116071428584 1214 24 3.6070498511904745 1214 25 0.029613095238104847 1214 26 0.21551339285714022 1214 27 3.4375372023809447 1214 28 3.517578125 1214 29 2.343824404761911 1214 30 1.1707961309523824 1214 31 3.057589285714286 1214 32 2.822451636904752 1214 33 1.7579055059523867 1214 34 0.14821428571428186 1214 35 2.8155505952380935 1214 36 -3.285230654761911 1214 37 1.9597656250000028 1214 38 -2.337165178571439 1214 39 1.030766369047626 1214 40 0.5152901785714334 1214 41 -2.630171130952391 1214 42 -3.1283110119047564 1214 43 0.963616071428568 1214 44 2.358165922619051 1214 45 2.7985305059523924 1214 46 -2.746075148809524 1214 47 1.2974888392857054 1214 48 -2.126506696428578 1214 49 0.3180059523809504 1214 50 3.417503720238095 1214 51 1.7303199404761926 1215 1 -2.6395275297619065 1215 2 1.2698288690476218 1215 3 1.5147879464285765 1215 4 1.6747209821428655 1215 5 -0.4882068452380892 1215 6 1.239508928571425 1215 7 1.848660714285721 1215 8 1.718508184523806 1215 9 1.612574404761908 1215 10 1.1809709821428456 1215 11 -0.35989583333333286 1215 12 3.4385230654761827 1215 13 1.6642485119047663 1215 14 1.0348586309523782 1215 15 2.2640625000000014 1215 16 1.8011904761904773 1215 17 2.167243303571418 1215 18 3.3707217261904745 1215 19 3.4249813988095212 1215 20 3.174293154761898 1215 21 0.5108630952380864 1215 22 2.8331845238095212 1215 23 2.6376116071428584 1215 24 3.1070498511904745 1215 25 3.279613095238105 1215 26 3.46551339285714 1215 27 2.9375372023809447 1215 28 4.017578125 1215 29 4.343824404761911 1215 30 2.6707961309523824 1215 31 2.557589285714286 1215 32 2.822451636904752 1215 33 2.2579055059523867 1215 34 1.6482142857142819 1215 35 3.3155505952380935 1215 36 -0.035230654761910785 1215 37 1.4597656250000028 1215 38 3.412834821428561 1215 39 3.780766369047626 1215 40 2.7652901785714334 1215 41 3.619828869047609 1215 42 2.6216889880952436 1215 43 1.713616071428568 1215 44 0.858165922619051 1215 45 2.2985305059523924 1215 46 3.503924851190476 1215 47 3.0474888392857054 1215 48 -2.126506696428578 1215 49 -3.1819940476190496 1215 50 1.417503720238095 1215 51 3.7303199404761926 1212 1 2.1104724702380935 1212 2 -3.480171130952378 1212 3 -3.2352120535714235 1212 4 -5.8252790178571345 1212 5 -0.23820684523808922 1212 6 -1.510491071428575 1212 7 -3.901339285714279 1212 8 2.218508184523806 1212 9 -0.8874255952380921 1212 10 1.6809709821428456 1212 11 0.8901041666666671 1212 12 -3.8114769345238173 1212 13 -0.0857514880952337 1212 14 -0.21514136904762182 1212 15 -5.985937499999999 1212 16 -5.948809523809523 1212 17 -6.082756696428582 1212 18 -4.3792782738095255 1212 19 0.9249813988095212 1212 20 1.174293154761898 1212 21 2.0108630952380864 1212 22 -3.9168154761904788 1212 23 1.1376116071428584 1212 24 0.6070498511904745 1212 25 -0.7203869047618952 1212 26 -5.28448660714286 1212 27 -2.0624627976190553 1212 28 -1.982421875 1212 29 -3.406175595238089 1212 30 -0.5792038690476176 1212 31 -7.192410714285714 1212 32 -1.927548363095248 1212 33 -0.2420944940476133 1212 34 1.1482142857142819 1212 35 0.8155505952380935 1212 36 -3.785230654761911 1212 37 -3.790234374999997 1212 38 -0.33716517857143913 1212 39 -4.469233630952374 1212 40 2.7652901785714334 1212 41 1.119828869047609 1212 42 2.1216889880952436 1212 43 2.713616071428568 1212 44 0.858165922619051 1212 45 0.2985305059523924 1212 46 -6.496075148809524 1212 47 3.0474888392857054 1212 48 1.623493303571422 1212 49 -6.68199404761905 1212 50 -3.832496279761905 1212 51 -9.269680059523807 1213 1 1.6104724702380935 1213 2 2.769828869047622 1213 3 1.0147879464285765 1213 4 -0.8252790178571345 1213 5 -1.4882068452380892 1213 6 2.5728422619047606 1213 7 3.848660714285721 1213 8 3.0518415178571416 1213 9 2.2792410714285722 1213 10 2.5143043154761813 1213 11 2.0567708333333314 1213 12 3.2718563988095184 1213 13 1.1642485119047663 1213 14 3.368191964285714 1213 15 3.7640625000000014 1213 16 2.4678571428571416 1213 17 3.0005766369047535 1213 18 3.20405505952381 1213 19 3.258314732142857 1213 20 3.5076264880952337 1213 21 2.844196428571422 1213 22 2.4998511904761855 1213 23 2.970944940476194 1213 24 2.773716517857139 1213 25 2.946279761904769 1213 26 3.1321800595238045 1213 27 3.7708705357142804 1213 28 2.517578125 1213 29 4.1771577380952465 1213 30 2.8374627976190467 1213 31 2.7242559523809504 1213 32 2.9891183035714164 1213 33 3.424572172619051 1213 34 3.9815476190476176 1213 35 3.648883928571429 1213 36 1.1314360119047535 1213 37 3.7930989583333385 1213 38 3.7461681547618966 1213 39 3.4474330357142904 1213 40 4.265290178571433 1213 41 3.9531622023809447 1213 42 2.788355654761908 1213 43 2.8802827380952323 1213 44 3.6914992559523867 1213 45 2.4651971726190567 1213 46 3.8372581845238116 1213 47 4.380822172619041 1213 48 4.456826636904758 1213 49 3.901339285714286 1213 50 3.7508370535714306 1213 51 3.2303199404761926 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 0.0 22 2.0 23 12.0 24 14.0 25 28.0 26 40.0 27 50.0 28 77.0 29 100.0 30 117.0 31 122.0 32 174.0 33 174.0 34 196.0 35 246.0 36 271.0 37 424.0 38 559.0 39 780.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.100974313551816 16.031886625332152 12.223206377325067 37.643932683790965 2 13.079421316799527 21.16917626217892 43.371715382344256 22.379687038677297 3 16.474756421612046 24.210215529967524 37.230587540596396 22.084440507824034 4 10.835547682314733 26.778860348390904 35.01623855919693 27.369353410097432 5 12.252731030410393 35.10481251845291 39.74018305284913 12.90227339828757 6 27.930321818718628 43.312666076173606 16.090935931502806 12.66607617360496 7 20.962503690581634 32.00472394449365 19.840566873339238 27.192205491585476 8 20.814880425155007 42.0726306465899 19.929140832595216 17.183348095659877 9 22.97018010038382 16.947150870977264 18.27576025981695 41.80690876882196 10 13.640389725420727 35.93150280484204 28.875110717449072 21.552996752288163 11 27.162680838500147 24.180690876882196 31.857100679067017 16.799527605550637 12 19.929140832595216 23.265426631237084 28.520814880425156 28.284617655742544 13 23.82639503985828 20.401535281960438 33.42190729258932 22.35016238559197 14 19.368172423974016 20.22438736344848 25.952170062001773 34.45527015057573 15 20.637732506643047 25.922645408916445 22.615884263359906 30.823737821080606 16 29.288455860643637 25.952170062001773 23.590197815175674 21.16917626217892 17 21.257750221434897 35.51815766164748 23.501623855919693 19.722468260997932 18 29.317980513728966 26.542663123708298 25.066430469441986 19.072925893120757 19 22.527310304103924 25.47977561263655 23.82639503985828 28.166519043401237 20 21.49394744611751 24.15116622379687 24.328314142308827 30.026572187776797 21 20.873929731325656 25.302627694124595 25.066430469441986 28.757012105107766 22 20.1358134041925 33.56953055801594 23.560673162090346 22.733982875701212 23 20.549158547387066 25.56834957189253 24.38736344847948 29.495128432240918 24 28.284617655742544 24.830233244759377 25.597874224977858 21.28727487452022 25 21.16917626217892 32.29997047534691 24.948331857100676 21.582521405373488 26 29.081783289046353 25.834071449660467 26.12931798051373 18.95482728077945 27 20.814880425155007 34.160023619722466 25.27310304103927 19.75199291408326 28 20.873929731325656 25.686448184233836 25.184529081783293 28.255093002657215 29 24.269264836138174 26.92648361381754 27.576025981694713 21.228225568349572 30 23.974018305284915 28.757012105107766 27.162680838500147 20.106288751107172 31 21.16917626217892 27.31030410392678 28.786536758193094 22.733982875701212 32 20.90345438441098 26.30646589902569 30.20372010628875 22.586359610274577 33 21.08060230292294 26.27694124594036 31.29613227044582 21.346324180690875 34 20.076764098021847 27.54650132860939 31.709477413640393 20.667257159728372 35 22.999704753469146 25.71597283731916 30.115146147032775 21.16917626217892 36 21.78919397697077 25.50930026572188 30.292294065544727 22.40921169176262 37 18.541482137584882 31.384706229701802 28.63891349276646 21.434898139946856 38 17.006200177147917 22.4977856510186 37.05343962208444 23.44257454974904 39 17.212872748745202 20.962503690581634 31.118984351933864 30.7056392087393 40 18.600531443755536 19.929140832595216 37.96870386772955 23.501623855919693 41 17.44906997342781 21.139651609093594 31.47328018895778 29.93799822852081 42 26.483613817537645 21.28727487452022 29.642751697667553 22.586359610274577 43 16.91762621789194 21.31679952760555 40.44877472689696 21.31679952760555 44 15.64806613522291 30.73516386182462 31.384706229701802 22.232063773250665 45 15.470918216710954 23.176852671981106 39.32683790965456 22.02539120165338 46 23.737821080602302 22.084440507824034 31.207558311189842 22.97018010038382 47 14.880425155004428 23.678771774431652 29.34750516681429 32.09329790374963 48 13.84706229701801 22.733982875701212 29.583702391496903 33.83525243578388 49 22.202539120165337 22.586359610274577 29.43607912607027 25.775022143489814 50 13.93563625627399 22.704458222615884 36.90581635665781 26.454089164452316 51 12.99084735754355 23.472099202834368 29.642751697667553 33.89430174195453 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 1.0 8 2.0 9 1.0 10 0.0 11 0.0 12 0.0 13 1.0 14 2.0 15 6.5 16 11.0 17 10.5 18 10.0 19 8.0 20 6.0 21 7.0 22 8.0 23 7.0 24 6.0 25 7.5 26 14.0 27 19.0 28 22.5 29 26.0 30 41.5 31 57.0 32 87.5 33 118.0 34 147.0 35 176.0 36 214.0 37 252.0 38 251.5 39 251.0 40 257.0 41 263.0 42 281.5 43 300.0 44 290.5 45 281.0 46 276.0 47 271.0 48 318.0 49 365.0 50 346.5 51 328.0 52 276.5 53 225.0 54 176.5 55 128.0 56 115.5 57 103.0 58 80.5 59 58.0 60 45.5 61 33.0 62 30.5 63 28.0 64 22.0 65 16.0 66 15.0 67 14.0 68 10.5 69 7.0 70 4.5 71 2.0 72 1.5 73 1.0 74 1.0 75 2.5 76 4.0 77 2.0 78 0.0 79 2.0 80 4.0 81 2.5 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 3387.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.49306170652494 #Duplication Level Percentage of deduplicated Percentage of total 1 98.92332789559543 89.51874815470919 2 0.35889070146818924 0.6495423678771775 3 0.13050570962479607 0.354295837023915 4 0.06525285481239804 0.23619722468260998 5 0.03262642740619902 0.14762326542663123 6 0.09787928221859706 0.5314437555358724 7 0.0 0.0 8 0.06525285481239804 0.47239444936521996 9 0.03262642740619902 0.2657218777679362 >10 0.2936378466557912 7.824033067611455 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC 50 1.4762326542663124 No Hit CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC 45 1.328609388839681 No Hit CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC 44 1.299084735754355 No Hit CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC 28 0.8266902863891349 No Hit CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC 27 0.7971656333038087 RNA PCR Primer, Index 39 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC 25 0.7381163271331562 RNA PCR Primer, Index 45 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC 20 0.5904930617065249 No Hit TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT 15 0.44286979627989376 No Hit CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC 11 0.3247711839385887 RNA PCR Primer, Index 23 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC 9 0.2657218777679362 No Hit TCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGT 8 0.23619722468260998 No Hit CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC 8 0.23619722468260998 No Hit CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC 6 0.1771479185119575 No Hit TTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT 6 0.1771479185119575 No Hit TCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGT 6 0.1771479185119575 No Hit CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC 5 0.14762326542663123 RNA PCR Primer, Index 27 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC 4 0.11809861234130499 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4 0.11809861234130499 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.029524653085326247 22 0.0 0.0 0.029524653085326247 23 0.0 0.0 0.029524653085326247 24 0.0 0.0 0.029524653085326247 25 0.0 0.0 0.029524653085326247 26 0.0 0.0 0.059049306170652495 27 0.0 0.0 0.08857395925597875 28 0.0 0.0 0.29524653085326247 29 0.0 0.0 0.7381163271331562 30 0.0 0.0 1.4762326542663124 31 0.0 0.0 2.4800708591674048 32 0.0 0.0 3.808680248007086 33 0.0 0.0 5.19633894301742 34 0.0 0.0 6.4954236787717745 35 0.0 0.0 7.7059344552701505 36 0.0 0.0 10.008857395925597 37 0.0 0.0 11.868910540301151 38 0.0 0.0 14.496604664895187 39 0.0 0.0 16.858576911721286 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTC 35 4.586689E-6 38.571426 45 GTATGCC 40 1.147836E-5 33.75 42 ATCTCCG 40 1.147836E-5 33.75 10 GCCCACG 40 1.147836E-5 33.75 18 TATGCCG 40 1.147836E-5 33.75 43 CATCTCC 40 1.147836E-5 33.75 9 GAGCCCA 40 1.147836E-5 33.75 16 ACATCTC 40 1.147836E-5 33.75 8 TCTCGTA 40 1.147836E-5 33.75 38 ATGCCGT 40 1.147836E-5 33.75 44 CGTATGC 40 1.147836E-5 33.75 41 CGAGCCC 40 1.147836E-5 33.75 15 CCGAGCC 40 1.147836E-5 33.75 14 TCGTATG 40 1.147836E-5 33.75 40 TCCGAGC 40 1.147836E-5 33.75 13 CTCGTAT 40 1.147836E-5 33.75 39 ATCTCGT 35 2.2682433E-4 32.142857 37 CCACGAG 35 2.2682433E-4 32.142857 20 CACGAGA 35 2.2682433E-4 32.142857 21 CCCACGA 35 2.2682433E-4 32.142857 19 >>END_MODULE