FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_92.34100000005491.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_92.34100000005491.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451053
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAGA5480.12149348302749345No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTC4980.11040831121841557No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGGC352.8180052E-432.1422238
TGCCGTC957.385097E-1026.05211845
TATGCCG952.1085725E-823.68374443
GTTATAT1003.62852E-822.502051
ATGCCGT1104.0363375E-922.49955644
ACGAGAC1156.7429937E-921.52131522
ATCTCGT1109.9524186E-820.45414237
TTACTAC550.00393333720.4541423
GTGTAAG1009.0220055E-720.2518461
CTCGTAT1151.590306E-719.5648339
CACGAGA1151.590306E-719.5648321
GGACAGT706.975397E-419.2853346
AACGCAG6650.018.9469956
ACAGTAC600.006505246718.749638
CTGACCG600.006505246718.749634
GTATCAA7300.018.4948371
GTCTTAG750.001108124218.001641
CCCACGA1253.821242E-717.99964519
TCTCGTA1253.821242E-717.99964538
AATCTCG1053.1061754E-517.14251936
Produced by FastQC (version 0.11.2)