FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_91.34100000005484.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_91.34100000005484.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences698908
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTA250.002106594435.9998329
TTAGGCG453.4890207E-529.9998579
GACCCGG551.367498E-424.5453366
TATGCCG651.5226968E-524.23065643
TGCCGTC651.5226968E-524.23065645
CCGTACC500.002263791622.49989340
CGTACCT500.002263791622.49989341
ATTATAC1459.094947E-1221.7240353
GTATTGG3100.021.0528051
GGCGTTT654.2360765E-420.76913312
TATACTA1109.9706085E-820.4544475
CGATTAA908.243995E-619.99990533
GAGATCG908.243995E-619.9999059
GTATCAA4750.019.8989121
AGGCGTT706.979945E-419.28562411
TAGGCGT706.979945E-419.28562410
ATCTCCG2750.018.81809245
AAATTCG2750.018.81809214
ATCGGAC600.006508183718.74990816
GGTTCGT851.203628E-418.52932418