##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 18h_90.34100000005477.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3374 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.35743924125667 38.0 32.0 38.0 32.0 38.0 2 35.46769413159455 38.0 32.0 38.0 32.0 38.0 3 35.327504445761704 38.0 32.0 38.0 32.0 38.0 4 35.56283343212804 38.0 32.0 38.0 32.0 38.0 5 35.610847658565504 38.0 32.0 38.0 32.0 38.0 6 37.46799051570836 38.0 38.0 40.0 32.0 40.0 7 37.14730290456431 38.0 38.0 40.0 32.0 40.0 8 37.15441612329579 38.0 38.0 40.0 32.0 40.0 9 37.03823355068168 38.0 38.0 40.0 32.0 40.0 10 37.072021339656196 38.0 38.0 40.0 32.0 40.0 11 37.15649081209247 38.0 38.0 40.0 32.0 40.0 12 36.930053349140486 38.0 38.0 40.0 32.0 40.0 13 36.95465323058684 38.0 38.0 40.0 32.0 40.0 14 36.76882039122703 38.0 38.0 40.0 32.0 40.0 15 36.97273266152934 38.0 38.0 40.0 32.0 40.0 16 36.844694724362775 38.0 38.0 40.0 32.0 40.0 17 36.80735032602252 38.0 38.0 40.0 32.0 40.0 18 36.78660343805572 38.0 38.0 40.0 32.0 40.0 19 36.8841138114997 38.0 38.0 40.0 32.0 40.0 20 36.74570243034973 38.0 38.0 40.0 32.0 40.0 21 36.77237700059277 38.0 38.0 40.0 32.0 40.0 22 36.77148784825133 38.0 38.0 40.0 32.0 40.0 23 36.882928275044456 38.0 38.0 40.0 32.0 40.0 24 36.883224659158266 38.0 38.0 40.0 32.0 40.0 25 36.85240071132187 38.0 38.0 40.0 32.0 40.0 26 36.76407824540605 38.0 38.0 40.0 32.0 40.0 27 36.7628927089508 38.0 38.0 40.0 32.0 40.0 28 36.75103734439834 38.0 38.0 40.0 32.0 40.0 29 36.72110254890338 38.0 38.0 40.0 32.0 40.0 30 36.658269116775344 38.0 38.0 40.0 32.0 40.0 31 36.622999407231774 38.0 38.0 40.0 32.0 40.0 32 36.72199170124481 38.0 38.0 40.0 32.0 40.0 33 36.74066390041494 38.0 38.0 40.0 32.0 40.0 34 36.49021932424422 38.0 38.0 40.0 32.0 40.0 35 36.628630705394194 38.0 38.0 40.0 32.0 40.0 36 36.63366923532899 38.0 38.0 40.0 32.0 40.0 37 36.52045050385299 38.0 38.0 40.0 32.0 40.0 38 36.57291049199763 38.0 38.0 40.0 32.0 40.0 39 36.440130409010074 38.0 38.0 40.0 27.0 40.0 40 36.40752815649081 38.0 38.0 40.0 27.0 40.0 41 36.477771191464136 38.0 38.0 40.0 32.0 40.0 42 36.38529934795495 38.0 38.0 40.0 27.0 40.0 43 36.36633076467101 38.0 38.0 40.0 27.0 40.0 44 36.07439241256669 38.0 38.0 40.0 27.0 40.0 45 36.01363366923533 38.0 38.0 40.0 27.0 40.0 46 35.91671606401897 38.0 38.0 40.0 27.0 40.0 47 35.719917012448136 38.0 38.0 40.0 27.0 40.0 48 35.79223473621814 38.0 38.0 40.0 27.0 40.0 49 35.77119146413752 38.0 38.0 40.0 27.0 40.0 50 35.797273266152935 38.0 38.0 40.0 27.0 40.0 51 34.96828689982217 38.0 32.0 40.0 13.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2201 1 1.5787202380952436 2201 2 -0.09211309523809774 2201 3 1.2497023809523924 2201 4 0.9835565476190595 2201 5 -0.6194568452380906 2201 6 0.575409226190466 2201 7 0.4288318452380935 2201 8 -3.6387276785714135 2201 9 -0.7157366071428584 2201 10 -4.102529761904755 2201 11 0.6876860119047592 2201 12 -2.0860119047619037 2201 13 0.6234002976190496 2201 14 -0.8274553571428527 2201 15 -0.564694940476187 2201 16 2.5369419642857167 2201 17 1.1492559523809618 2201 18 0.2362351190476204 2201 19 1.032440476190466 2201 20 1.1547247023809533 2201 21 1.094456845238092 2201 22 -0.34892113095237676 2201 23 -5.109151785714289 2201 24 -3.3228422619047677 2201 25 -1.6646577380952294 2201 26 -3.2725818452380935 2201 27 -3.335677083333323 2201 28 -4.010267857142857 2201 29 -3.502976190476197 2201 30 -1.6001860119047606 2201 31 -3.4676339285714377 2201 32 -2.9139136904761855 2201 33 -2.9175967261904816 2201 34 -4.605096726190467 2201 35 -3.3907738095238074 2201 36 0.19497767857143344 2201 37 -2.7979538690476176 2201 38 0.30483630952380736 2201 39 -3.2668154761904873 2201 40 3.274888392857143 2201 41 2.931659226190483 2201 42 -5.127194940476194 2201 43 1.7183407738095298 2201 44 2.032031250000003 2201 45 3.2925595238095227 2201 46 -4.531584821428567 2201 47 -2.5340773809523824 2201 48 -1.4450520833333442 2201 49 -2.727269345238092 2201 50 -2.227827380952384 2201 51 -2.9945312500000014 1101 1 -0.5212797619047578 1101 2 0.2578869047619037 1101 3 1.5497023809523895 1101 4 1.2835565476190567 1101 5 -0.019456845238089215 1101 6 -2.624590773809537 1101 7 -4.471168154761905 1101 8 2.4112723214285836 1101 9 -0.7157366071428584 1101 10 1.9474702380952422 1101 11 0.38768601190476204 1101 12 1.563988095238095 1101 13 1.5234002976190482 1101 14 1.9725446428571445 1101 15 1.9853050595238102 1101 16 -0.4630580357142833 1101 17 2.449255952380959 1101 18 2.386235119047619 1101 19 3.1324404761904674 1101 20 1.6547247023809533 1101 21 1.9944568452380906 1101 22 -2.5489211309523796 1101 23 2.0408482142857096 1101 24 1.5271577380952337 1101 25 2.3853422619047677 1101 26 0.5774181547619079 1101 27 2.3143229166666757 1101 28 1.739732142857143 1101 29 2.5470238095238003 1101 30 2.4498139880952365 1101 31 2.982366071428565 1101 32 2.6360863095238116 1101 33 3.0324032738095212 1101 34 2.9449032738095298 1101 35 3.0592261904761955 1101 36 1.5449776785714349 1101 37 2.852046130952381 1101 38 2.8548363095238045 1101 39 1.5831845238095141 1101 40 3.474888392857146 1101 41 3.131659226190486 1101 42 3.322805059523809 1101 43 2.9183407738095326 1101 44 2.1320312500000043 1101 45 3.4925595238095255 1101 46 -3.5815848214285637 1101 47 1.9159226190476204 1101 48 3.0049479166666586 1101 49 3.322730654761905 1101 50 2.272172619047616 1101 51 3.5554687499999957 2202 1 -2.9212797619047564 2202 2 -5.742113095238096 2202 3 2.7497023809523924 2202 4 0.08355654761906095 2202 5 -1.219456845238092 2202 6 -4.0245907738095354 2202 7 -0.6711681547619079 2202 8 -0.1887276785714107 2202 9 2.084263392857139 2202 10 -0.8525297619047549 2202 11 -1.8123139880952408 2202 12 -4.0360119047619065 2202 13 -0.6765997023809476 2202 14 -0.8274553571428527 2202 15 -4.614694940476184 2202 16 -3.0630580357142847 2202 17 -2.9507440476190396 2202 18 -8.81376488095238 2202 19 -0.46755952380953403 2202 20 -5.745275297619045 2202 21 -9.605543154761907 2202 22 -5.548921130952376 2202 23 -2.9591517857142904 2202 24 0.12715773809523512 2202 25 -16.814657738095228 2202 26 -6.422581845238092 2202 27 -9.885677083333324 2202 28 -1.8602678571428584 2202 29 2.1470238095238017 2202 30 -0.7501860119047592 2202 31 -7.817633928571439 2202 32 -14.963913690476186 2202 33 -11.167596726190482 2202 34 -5.855096726190467 2202 35 -6.140773809523807 2202 36 1.1449776785714363 2202 37 -10.547953869047618 2202 38 -15.145163690476192 2202 39 -2.416815476190486 2202 40 -2.3251116071428584 2202 41 1.931659226190483 2202 42 -2.877194940476194 2202 43 0.5183407738095269 2202 44 -8.267968749999998 2202 45 -4.707440476190477 2202 46 -5.981584821428566 2202 47 -13.084077380952383 2202 48 -3.395052083333347 2202 49 -12.077269345238093 2202 50 -4.327827380952385 2202 51 -2.844531250000003 1102 1 2.2215773809523895 1102 2 2.6578869047619023 1102 3 1.8925595238095383 1102 4 2.4835565476190595 1102 5 2.3805431547619094 1102 6 2.575409226190466 1102 7 2.0716889880952394 1102 8 2.7541294642857324 1102 9 2.998549107142857 1102 10 2.8617559523809604 1102 11 3.187686011904759 1102 12 3.1639880952380963 1102 13 2.837686011904765 1102 14 2.8868303571428626 1102 15 3.185305059523813 1102 16 3.251227678571432 1102 17 2.506398809523816 1102 18 3.7005208333333357 1102 19 2.9610119047618966 1102 20 2.5118675595238074 1102 21 2.1658854166666615 1102 22 3.3653645833333385 1102 23 3.0694196428571416 1102 24 3.355729166666663 1102 25 3.013913690476201 1102 26 3.405989583333337 1102 27 3.6286086309523924 1102 28 3.4540178571428584 1102 29 3.4613095238095184 1102 30 3.3640997023809547 1102 31 3.4966517857142776 1102 32 3.5503720238095298 1102 33 3.8324032738095184 1102 34 3.859188988095248 1102 35 3.573511904761908 1102 36 3.9449776785714334 1102 37 4.452046130952382 1102 38 3.7691220238095227 1102 39 3.4117559523809433 1102 40 3.9891741071428584 1102 41 3.074516369047629 1102 42 2.6942336309523753 1102 43 3.1469122023809604 1102 44 3.1034598214285722 1102 45 -1.8502976190476232 1102 46 -0.49587053571428186 1102 47 0.28735119047618696 1102 48 -0.6236235119047748 1102 49 0.37987351190476204 1102 50 1.2721726190476161 1102 51 0.326897321428568 2203 1 1.8787202380952408 2203 2 1.4578869047618994 2203 3 0.3497023809523938 2203 4 0.08355654761906095 2203 5 2.3805431547619094 2203 6 2.1754092261904674 2203 7 -3.2711681547619094 2203 8 -1.7887276785714121 2203 9 -0.9157366071428612 2203 10 1.9474702380952422 2203 11 1.9876860119047564 2203 12 0.16398809523809632 2203 13 0.723400297619051 2203 14 -2.62745535714285 2203 15 -3.01469494047619 2203 16 0.5369419642857167 2203 17 -2.550744047619041 2203 18 -1.8137648809523768 2203 19 0.532440476190466 2203 20 -1.7452752976190453 2203 21 1.9944568452380906 2203 22 2.851078869047626 2203 23 3.2408482142857125 2203 24 3.5271577380952337 2203 25 2.7853422619047734 2203 26 1.9774181547619065 2203 27 -2.2856770833333258 2203 28 2.939732142857146 2203 29 1.3470238095238045 2203 30 -2.1501860119047578 2203 31 -2.017633928571435 2203 32 -2.3639136904761884 2203 33 -1.9675967261904788 2203 34 0.34490327380953545 2203 35 -5.140773809523807 2203 36 0.14497767857143629 2203 37 3.2520461309523796 2203 38 0.2548363095238102 2203 39 -1.8168154761904844 2203 40 1.6748883928571416 2203 41 -3.068340773809517 2203 42 0.3228050595238088 2203 43 -0.0816592261904674 2203 44 -1.66796875 2203 45 -1.107440476190476 2203 46 -1.9815848214285694 2203 47 1.315922619047619 2203 48 -1.9950520833333414 2203 49 2.9227306547619065 2203 50 1.472172619047619 2203 51 2.35546875 1103 1 1.0787202380952436 1103 2 -0.17544642857143344 1103 3 -0.2502976190476076 1103 4 -0.3497767857142762 1103 5 -0.6194568452380906 1103 6 1.2420758928571303 1103 7 1.9288318452380935 1103 8 2.944605654761922 1103 9 1.6175967261904773 1103 10 2.147470238095245 1103 11 1.521019345238095 1103 12 0.16398809523809632 1103 13 2.1234002976190496 1103 14 1.1725446428571473 1103 15 0.8519717261904773 1103 16 -1.9630580357142833 1103 17 -1.5174107142857025 1103 18 -1.5137648809523796 1103 19 0.3657738095238017 1103 20 -1.511941964285711 1103 21 -2.905543154761908 1103 22 0.48441220238095895 1103 23 0.14084821428571104 1103 24 -1.239508928571432 1103 25 1.0853422619047706 1103 26 2.3107514880952422 1103 27 2.247656250000013 1103 28 1.739732142857143 1103 29 3.080357142857139 1103 30 2.983147321428575 1103 31 2.4490327380952266 1103 32 -5.497247023809518 1103 33 1.8324032738095184 1103 34 -0.8550967261904674 1103 35 0.5258928571428569 1103 36 -0.5550223214285666 1103 37 2.1187127976190467 1103 38 2.388169642857143 1103 39 2.649851190476177 1103 40 2.9415550595238074 1103 41 2.931659226190483 1103 42 2.122805059523806 1103 43 3.385007440476194 1103 44 3.198697916666667 1103 45 2.959226190476187 1103 46 1.5517485119047691 1103 47 3.382589285714282 1103 48 2.1382812499999915 1103 49 3.522730654761908 1103 50 3.272172619047616 1103 51 3.422135416666663 2204 1 -0.49270833333332575 2204 2 0.943601190476187 2204 3 1.8925595238095383 2204 4 0.05498511904762893 2204 5 1.5234002976190553 2204 6 1.7182663690476119 2204 7 2.0716889880952394 2204 8 -0.960156249999983 2204 9 -1.001450892857143 2204 10 0.5760416666666757 2204 11 -1.526599702380956 2204 12 1.7354166666666657 2204 13 -3.44802827380952 2204 14 0.8868303571428626 2204 15 -3.957552083333333 2204 16 -0.6059151785714292 2204 17 -2.2078869047618923 2204 18 -4.01376488095238 2204 19 -1.7532738095238187 2204 20 -0.7738467261904773 2204 21 -1.6912574404761926 2204 22 1.936793154761908 2204 23 2.497991071428565 2204 24 2.212872023809517 2204 25 2.15677083333334 2204 26 3.691703869047622 2204 27 3.057180059523823 2204 28 2.882589285714289 2204 29 2.032738095238088 2204 30 3.07838541666667 2204 31 2.068080357142847 2204 32 0.26465773809524507 2204 33 1.8324032738095184 2204 34 2.1449032738095326 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1214 10 -0.6025297619047549 1214 11 -0.5623139880952408 1214 12 -0.08601190476190368 1214 13 2.6234002976190496 1214 14 0.17254464285714732 1214 15 2.185305059523813 1214 16 0.5369419642857167 1214 17 -3.600744047619038 1214 18 -5.26376488095238 1214 19 -6.967559523809534 1214 20 0.6547247023809533 1214 21 -6.905543154761908 1214 22 -4.098921130952377 1214 23 -6.859151785714289 1214 24 -3.8228422619047677 1214 25 -0.16465773809522943 1214 26 -7.0225818452380935 1214 27 -6.585677083333323 1214 28 -1.010267857142857 1214 29 -4.502976190476197 1214 30 -2.1001860119047606 1214 31 2.2823660714285623 1214 32 0.33608630952381446 1214 33 -3.4175967261904816 1214 34 2.6449032738095326 1214 35 -3.8907738095238074 1214 36 0.19497767857143344 1214 37 -3.2979538690476176 1214 38 2.0548363095238074 1214 39 -4.766815476190487 1214 40 1.274888392857143 1214 41 2.931659226190483 1214 42 -3.127194940476194 1214 43 -6.78165922619047 1214 44 -3.717968749999997 1214 45 -9.707440476190477 1214 46 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1215 32 2.6360863095238116 1215 33 -2.3675967261904844 1215 34 -0.4550967261904688 1215 35 -1.1407738095238074 1215 36 -1.8550223214285637 1215 37 -1.7479538690476204 1215 38 0.45483630952380594 1215 39 -2.21681547619049 1215 40 -1.9251116071428598 1215 41 -3.868340773809514 1215 42 0.7228050595238074 1215 43 -2.481659226190473 1215 44 3.33203125 1215 45 3.4925595238095255 1215 46 3.4184151785714363 1215 47 2.315922619047619 1215 48 2.604947916666653 1215 49 1.7227306547619108 1215 50 0.27217261904761614 1215 51 3.155468749999997 1212 1 1.5787202380952436 1212 2 1.1578869047619023 1212 3 1.2497023809523924 1212 4 0.9835565476190595 1212 5 0.8805431547619094 1212 6 1.075409226190466 1212 7 1.4288318452380935 1212 8 1.6112723214285865 1212 9 1.7842633928571416 1212 10 1.147470238095245 1212 11 1.1876860119047592 1212 12 0.16398809523809632 1212 13 2.1234002976190496 1212 14 2.6725446428571473 1212 15 2.185305059523813 1212 16 1.5369419642857167 1212 17 1.6492559523809618 1212 18 2.9862351190476204 1212 19 2.032440476190466 1212 20 2.6547247023809533 1212 21 -0.15554315476190794 1212 22 1.6510788690476232 1212 23 2.640848214285711 1212 24 -1.0728422619047677 1212 25 1.5853422619047706 1212 26 0.4774181547619065 1212 27 1.914322916666677 1212 28 2.739732142857143 1212 29 2.747023809523803 1212 30 1.1498139880952394 1212 31 1.7823660714285623 1212 32 0.8360863095238145 1212 33 2.3324032738095184 1212 34 2.6449032738095326 1212 35 1.3592261904761926 1212 36 0.9449776785714334 1212 37 1.9520461309523824 1212 38 2.0548363095238074 1212 39 3.4831845238095127 1212 40 3.274888392857143 1212 41 3.431659226190483 1212 42 2.622805059523806 1212 43 3.2183407738095298 1212 44 3.032031250000003 1212 45 2.7925595238095227 1212 46 3.2184151785714334 1212 47 3.7159226190476176 1212 48 0.8049479166666558 1212 49 3.022730654761908 1212 50 1.2721726190476161 1212 51 -2.9945312500000014 1213 1 -5.921279761904756 1213 2 1.1578869047619023 1213 3 1.2497023809523924 1213 4 -5.2664434523809405 1213 5 2.3805431547619094 1213 6 -4.674590773809534 1213 7 1.9288318452380935 1213 8 -4.1387276785714135 1213 9 2.2842633928571416 1213 10 0.14747023809524507 1213 11 -1.3123139880952408 1213 12 -7.336011904761904 1213 13 -10.87659970238095 1213 14 -4.077455357142853 1213 15 -7.314694940476187 1213 16 -6.213058035714283 1213 17 -13.100744047619038 1213 18 -11.51376488095238 1213 19 -6.967559523809534 1213 20 -3.3452752976190467 1213 21 -5.655543154761908 1213 22 -3.3489211309523768 1213 23 -0.859151785714289 1213 24 -3.3228422619047677 1213 25 -2.1646577380952294 1213 26 -6.5225818452380935 1213 27 -6.585677083333323 1213 28 0.7397321428571431 1213 29 -4.002976190476197 1213 30 -7.100186011904761 1213 31 -16.467633928571438 1213 32 -6.6639136904761855 1213 33 -0.4175967261904816 1213 34 -5.605096726190467 1213 35 0.8592261904761926 1213 36 -6.555022321428567 1213 37 -0.04795386904761756 1213 38 -7.945163690476193 1213 39 -12.766815476190487 1213 40 -3.475111607142857 1213 41 -1.818340773809517 1213 42 -5.627194940476194 1213 43 -6.78165922619047 1213 44 -6.217968749999997 1213 45 -9.707440476190477 1213 46 2.7184151785714334 1213 47 -4.534077380952382 1213 48 -5.445052083333344 1213 49 0.022730654761907942 1213 50 -4.977827380952384 1213 51 0.5054687499999986 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 2.0 21 2.0 22 3.0 23 5.0 24 18.0 25 18.0 26 31.0 27 41.0 28 64.0 29 81.0 30 105.0 31 107.0 32 135.0 33 124.0 34 171.0 35 194.0 36 239.0 37 355.0 38 557.0 39 1122.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.78048056956393 16.760605161673094 12.399881340848413 31.059032927914565 2 15.056312981624185 21.16182572614108 39.27089508002371 24.510966212211024 3 19.294605809128633 22.228808535862477 31.387077652637817 27.089508002371073 4 14.344991108476584 20.03556609365738 32.15767634854772 33.46176644931831 5 15.649081209247184 33.995257854179016 35.773562537048015 14.582098399525787 6 32.424422050978066 36.39596917605216 16.212211025489033 14.967397747480735 7 27.237700059276822 30.64611736810907 20.18375815056313 21.93242442205098 8 24.244220509780675 37.492590397154714 19.08713692946058 19.176052163604034 9 26.526378186129225 16.390041493775932 18.642560758743333 38.44101956135152 10 17.160640189685832 29.90515708358032 29.045643153526974 23.888559573206877 11 34.084173088322466 22.199170124481327 24.629519857735627 19.08713692946058 12 22.258446947243627 24.570243034973323 28.42323651452282 24.748073503260226 13 28.15649081209247 22.169531713100177 27.860106698280973 21.81387077652638 14 22.228808535862477 20.569057498518077 26.111440426793127 31.09069353882632 15 23.621813870776524 27.711914641375223 22.110254890337877 26.556016597510375 16 26.793123888559574 25.311203319502074 25.311203319502074 22.58446947243628 17 22.080616478956728 28.48251333728512 26.318909306461176 23.117960877296976 18 25.518672199170123 25.874333135743928 26.674570243034974 21.93242442205098 19 24.036751630112626 24.540604623592174 26.111440426793127 25.311203319502074 20 23.829282750444577 24.777711914641376 26.763485477178424 24.629519857735627 21 22.37700059276823 27.089508002371073 26.793123888559574 23.740367516301124 22 23.562537048014224 27.830468286899823 25.75577949021932 22.851215174866628 23 22.55483106105513 24.748073503260226 27.29697688203912 25.400118553645523 24 25.75577949021932 24.836988737403676 26.733847065797274 22.67338470657973 25 22.31772377000593 28.12685240071132 26.585655008891525 22.969768820391227 26 25.518672199170123 25.548310610551273 27.622999407231774 21.310017783046828 27 22.969768820391227 28.48251333728512 26.052163604030827 22.49555423829283 28 22.139893301719027 25.78541790160048 26.407824540604626 25.666864256075872 29 23.058684054534677 27.267338470657972 26.644931831653825 23.029045643153527 30 22.258446947243627 27.089508002371073 28.42323651452282 22.228808535862477 31 23.799644339063423 25.637225844694722 27.800829875518673 22.762299940723178 32 21.78423236514523 24.807350326022526 29.342027267338473 24.066390041493776 33 22.762299940723178 25.84469472436278 28.30468286899822 23.088322465915827 34 24.214582098399525 24.392412566686424 31.00177830468287 20.391227030231178 35 23.236514522821576 23.888559573206877 30.58684054534677 22.28808535862478 36 22.762299940723178 25.518672199170123 28.71962062833432 22.999407231772377 37 21.93242442205098 27.860106698280973 27.889745109662123 22.31772377000593 38 21.962062833432128 22.791938352104328 32.809721398933014 22.43627741553053 39 21.280379371665678 23.858921161825727 29.104919976289274 25.75577949021932 40 21.04327208061648 22.880853586247778 33.60995850622407 22.46591582691168 41 21.398933017190277 23.266152934202726 28.008298755186722 27.32661529342027 42 23.918197984588026 22.851215174866628 30.55720213396562 22.67338470657973 43 20.598695909899227 23.325429756965026 32.15767634854772 23.918197984588026 44 19.73918197984588 28.54179016004742 29.66804979253112 22.050978067575578 45 19.73918197984588 24.510966212211024 33.43212803793716 22.31772377000593 46 23.295791345583876 24.659158269116777 28.21576763485477 23.829282750444577 47 18.583283935981033 25.044457617071725 28.867812685240068 27.504445761707174 48 18.731475992886782 24.748073503260226 28.51215174866627 28.008298755186722 49 21.54712507409603 24.451689389448724 29.460580912863072 24.540604623592174 50 18.405453467694134 24.273858921161825 31.387077652637817 25.933609958506228 51 18.90930646117368 24.836988737403676 28.21576763485477 28.037937166567872 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 1.0 4 2.0 5 3.5 6 5.0 7 4.0 8 3.0 9 3.0 10 3.0 11 5.0 12 7.0 13 13.5 14 20.0 15 17.0 16 14.0 17 25.0 18 36.0 19 29.5 20 23.0 21 23.5 22 24.0 23 20.0 24 16.0 25 13.5 26 11.5 27 12.0 28 18.5 29 25.0 30 38.0 31 51.0 32 71.5 33 92.0 34 117.0 35 142.0 36 152.5 37 163.0 38 183.0 39 203.0 40 217.0 41 231.0 42 225.5 43 220.0 44 227.5 45 235.0 46 220.0 47 205.0 48 239.0 49 273.0 50 255.5 51 238.0 52 234.5 53 231.0 54 203.0 55 175.0 56 176.0 57 177.0 58 160.0 59 143.0 60 127.0 61 111.0 62 100.5 63 90.0 64 76.5 65 63.0 66 56.0 67 49.0 68 39.5 69 30.0 70 23.0 71 16.0 72 14.0 73 12.0 74 10.5 75 7.0 76 5.0 77 4.5 78 4.0 79 4.5 80 5.0 81 2.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08891523414344991 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 3374.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.7320687611144 #Duplication Level Percentage of deduplicated Percentage of total 1 98.63777089783282 94.4279786603438 2 0.9287925696594427 1.7783046828689981 3 0.09287925696594426 0.26674570243034973 4 0.12383900928792571 0.4742145820983995 5 0.09287925696594426 0.44457617071724953 6 0.030959752321981428 0.17783046828689983 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.09287925696594426 2.430349733254298 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC 47 1.3930053349140488 RNA PCR Primer, Index 45 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC 20 0.5927682276229994 No Hit CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC 15 0.44457617071724953 RNA PCR Primer, Index 23 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC 6 0.17783046828689983 No Hit CTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTC 5 0.14819205690574985 No Hit CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC 5 0.14819205690574985 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.14819205690574985 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC 4 0.11855364552459988 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4 0.11855364552459988 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC 4 0.11855364552459988 No Hit CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC 4 0.11855364552459988 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.02963841138114997 26 0.0 0.0 0.02963841138114997 27 0.0 0.0 0.05927682276229994 28 0.0 0.0 0.14819205690574985 29 0.0 0.0 0.3260225251926497 30 0.0 0.0 0.5927682276229994 31 0.0 0.0 0.918790752815649 32 0.0 0.0 1.4522821576763485 33 0.0 0.0 2.222880853586248 34 0.0 0.0 3.0527563722584468 35 0.0 0.0 3.971547125074096 36 0.0 0.0 4.8903378778897455 37 0.0 0.0 6.046235921754594 38 0.0 0.0 7.320687611144042 39 0.0 0.0 8.861885002963842 >>END_MODULE >>Kmer Content pass >>END_MODULE