FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_90.34100000005477.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_90.34100000005477.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3374
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC471.3930053349140488RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC200.5927682276229994No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC150.44457617071724953RNA PCR Primer, Index 23 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC60.17783046828689983No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTC50.14819205690574985No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC50.14819205690574985No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.14819205690574985No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC40.11855364552459988No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.11855364552459988No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC40.11855364552459988No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC40.11855364552459988No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)