FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_88.3410000000545e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_88.3410000000545e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241533
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGAT352.8139024E-432.1427115
TTTGCAC352.8139024E-432.142713
GTTTAGA300.005134218430.0060751
TAATAGT651.5173391E-524.2306584
GGTATCA2650.022.929171
CGTATAA602.4597652E-422.49989544
TGAGTAA754.583493E-520.999910
CATAATA754.583493E-520.99992
CTAGGAC654.2250188E-420.7691363
TAAGAGT550.003927782620.4544534
AATAGTG807.531731E-519.6874085
GCGTATA706.961845E-419.28562743
GTATCAA4400.018.9242861
TTGTACA600.00649613318.7499124
ATAATAC851.1994907E-418.5293273
CATACGC750.001106809517.99991638
TAGTATG901.8580542E-417.499925
GTCTAAG800.0017056216.87492235
GCATACG800.0017056216.87492237
AACGCAG4600.016.6303586
Produced by FastQC (version 0.11.2)