FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_87.34100000005441.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_87.34100000005441.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences984574
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTAG13810.14026370795897514No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCT11330.115075149252367No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTAT10480.10644197388921504No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACCG207.033614E-444.99893635
TGCCGTC1150.037.17492345
TAGCGCA250.00210720735.999159
CTAGTCG250.00210720735.9991520
ATGCCGT1300.032.8838444
CCACGAG1400.032.14209420
CCCACGA1450.031.03374719
TATGCCG1400.030.5349943
GCGCACA300.00514622330.00081311
GTGGGAC300.005147501429.99928923
GCCCACG1600.029.53055218
CGAGCCC1550.029.03304315
CATCCGA406.1663653E-428.1243349
ACGAGAC1650.027.27208122
CTAAACG957.421477E-1026.05333715
CCCTCAC2250.026.00070429
CTCGTAT1750.025.71367639
CACGAGA1750.025.71367621
GACGTCT1700.025.14774126
CCGAGCC1800.025.00067714
Produced by FastQC (version 0.11.2)