FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_86.34100000005434.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_86.34100000005434.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1177428
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAGA17080.14506194858624052No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCT16810.14276881473856576No Hit
GGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCTT15810.13427572641384442No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTAG15720.13351134846461948No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCGT352.8182805E-432.14701512
CACGAGA1450.026.37823921
TATGCCG1400.025.7132443
CGTACAC450.001226862424.9989873
TTACCGC551.3670296E-424.5486316
GCCCACG1500.024.00004418
TGCCGTC1500.024.00004445
CCCACGA1500.023.99902519
CGAGCCC1600.022.5029115
ACGACGG500.00226517622.4990886
ACGAGAC1600.022.49908622
ATGCCGT1650.021.81729744
AGACGTC1459.094947E-1221.7241825
CTCGTAT1700.021.17561139
TCTCGTA1700.021.17561138
CCACGAG1700.021.17561120
CATCTCC16700.020.61381145
TCGTATG1750.020.57059540
AATCGAC550.00393364620.45719116
ATACGGG550.00393751820.4537166
Produced by FastQC (version 0.11.2)