FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_85.34100000005427.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_85.34100000005427.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1688943
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC42190.24980120702711694No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAAC19980.11829884134633317No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4600.040.59806445
ATGGCGG651.4048055E-831.15310930
ATTCGGC300.0051485329.9992920
TATGCCG6700.027.87247543
ATGCCGT6800.027.46258544
ACGAGAC6850.027.26212922
AGACGTC6300.027.14221225
CCACGAG7100.026.6190920
TATCTCG6750.026.3327136
GCCCACG7100.026.30297318
CACGAGA7350.025.71367621
CCCACGA7350.025.4075619
ATCTCGT7300.025.27337337
GCGGGGT901.19216566E-824.99940744
GACGTCT6950.024.92746726
CGTCTTA7050.024.25474528
ACGTCTT7150.024.23019627
TCTCGTA7850.023.50262838
CGAGACG7950.023.4900123
CTCGTAT7950.023.20699739
Produced by FastQC (version 0.11.2)