FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_84.3410000000541a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_84.3410000000541a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631393
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCT8670.13731542795057913No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTAG7800.12353637116661097No Hit
GGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCTT6890.10912379453050636No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAGA6880.10896541456747223No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC6540.10358049582431228No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC900.039.99908445
AGACGTC855.456968E-1231.76649525
AACCCGA300.00514609729.9993156
AAAGCGT602.4165456E-729.99931510
GCCCACG1150.029.34715718
TATGCCG1150.029.34715743
GACGTCT952.3646862E-1128.42265326
ATCCTAC3200.027.4212476
CTCGTAT1250.026.99938239
CCCACGA1250.026.99938219
AAACCCG507.154491E-526.999385
TATCTCG1200.026.24940136
CGAGACG1200.026.24940123
ACGAGAC1200.026.24940122
CACGAGA1300.025.96094521
GCGTTCA709.2982555E-725.713713
CCACGAG1400.025.713720
CCCAATT1850.025.53995513
CGGTTAG450.001226106124.99942832
TCTCGTA1350.024.99942838
Produced by FastQC (version 0.11.2)