FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_83.3410000000540d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_83.3410000000540d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947753
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC11110.1172246355326757No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1500.038.99913445
GTATCGC352.8203745E-432.14214333
ACTGCGT300.005147368629.999338
TAGTCGG300.005147368629.9993320
TATGCCG2250.025.9994243
CGTCTTA2300.025.43555828
CGCCACT450.001226555424.99944337
CGATGCA651.5236477E-524.23022833
GCCCACG2450.023.8770218
ATGCCGT2450.023.8770244
GACGTCT2700.022.50068726
TAAGTAA500.002264612122.4994985
CCCACGA2600.022.49949819
CGAGGTT500.002264612122.4994985
TTCGGGG802.974417E-622.49949823
TATCTCG2650.022.0749836
CGAGACG2650.022.0749823
TTTCGGG955.530801E-721.31531322
GTGTCCG1201.1048542E-820.624549
CCACGAG2950.020.59276220
Produced by FastQC (version 0.11.2)